ARUP's Laboratory Test Directory

Acanthamoeba and Naegleria Culture and Stains, CSF : 2002584

Mnemonic: ACANTCSF

Methodology: Qualitative Culture/Microscopy/Calcofluor Stain/Giemsa Stain
Performed: Sun-Sat
Reported: 2-10 days
Specimen Required: Collect: CSF.

Specimen Preparation: Transfer 1 mL CSF to a sterile ARUP Standard Transport Tube (ARUP supply # 43115). Available online through eSupply using ARUP Connect™ or contact Client Services at (800) 522-2787. (Min: 0.5 mL)

Storage/Transport Temperature: Room temperature.

Unacceptable Conditions: Specimens in media or preservatives.

Stability (collection to initiation of testing): Ambient: 72 hours; Refrigerated: Unacceptable; Frozen: Unacceptable

Reference Interval:
Negative
Interpretive Data: The stains will detect free-living amoeba such as Acanthamoeba species, Naegleria fowleri, and Balamuthia mandrillaris.

The culture will detect free-living amoeba such as Acanthamoeba species and Naegleria fowleri, but will NOT detect Balamuthia mandrillaris.
Note: For all other specimen types refer to Acanthamoeba and Naegleria Culture (ARUP test code 0060245) and Amoeba Calcofluor Stain (ARUP test code 0060250). For Entamoeba histolytica detection refer to Entamoeba histolytica Antigen, EIA (ARUP test code 0058001).
CPT Code(s): 87081; 87206; 87207
Cross References: Naegleria Culture (Acanthamoeba and Naegleria Culture and Stains, CSF)