ARUP's Laboratory Test Directory

Chimerism, Post-Transplant, Sorted Cells : 2002064
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Cell Isolation Request for Chimerism, Post-Transplant, Sorted Cells
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Additional Technical Information


Mnemonic: STR-POSTSC

Ordering Recommendation: Monitor engraftment of donor cells post allogenic stem cell transplantation.
Methodology: Polymerase Chain Reaction/Fragment Analysis
Performed: Sun-Sat
Reported: 5-9 days
Specimen Required: Collect: Lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A or B). OR bone marrow.

Specimen Preparation: Transport 3 mL whole blood. (Min: 3 mL) OR 1 mL bone marrow. Ship overnight. If cell sorting is required, specimens should be received within 24 hours of collection for optimal isolation of the requested cell line(s). Increase the amount of blood/bone marrow submitted based on the number of cell lines requested and for patients with low cell counts.

Storage/Transport Temperature: Ambient.

Remarks: Post-transplantation genotypes will be compared to pre-transplant recipient and donor genotypes. Therefore, donor and recipient specimens must be obtained and genotyped before the transplant event occurs. Please provide the results and date of the patient's most recent WBC and differential counts. When submitting bone marrow specimens for cell sorting, please provide information regarding the general cellularity of the patient's bone marrow. See Cell Isolation Request for Chimerism, Post-Transplant, Sorted Cells.

Unacceptable Conditions: Frozen specimens. Clotted or hemolyzed specimens.

Stability (collection to initiation of testing): Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable
Reference Interval:
Type Donor - Donor cells only.
Type Recipient - Recipient cells only.
Mixed - Donor and Recipient cells present. Semi-quantitative results of percentage of donor and recipient cells will be reported.
Interpretive Data: Background Information for Chimerism, Post-Transplant, Sorted Cells:
Indication: Monitoring for bone marrow transplant patients; correlation with clinical status and consideration of the interval between bone marrow transplantation and testing is necessary for proper interpretation of results.
Methodology: PCR followed by capillary electrophoresis. Specimens are analyzed using 15 autosomal markers (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, THO1, D13S317, D16S539, D2S1338, D19S433, vWa, TPOX, D18S51, D5S818, and FGA) and one gender marker (amelogenin).
Kit Used: AmpFLSTR Identifiler® PCR Amplification Kit, Applied Biosystems.
Limit of Detection: 2 percent of minor cell population.

See Compliance Statement B: www.aruplab.com/CS
Note: If requesting multiple cell lines, additional charges apply and vary based on the cell line requested (BMT Cell Isolation or BMT Chimerism Sort). Chimerism, Post-Transplant, Sorted Cells will be billed as separate tests for each cell line requested.
CPT Code(s): 81268