ARUP's Laboratory Test Directory

Bordetella pertussis Antibodies, IgG and IgM by ELISA with Reflex to Immunoblot : 2001784

Mnemonic: BORDPAN

Ordering Recommendation: If serology is used to assess late-stage pertussis, the recommended test is Bordetella pertussis Antibodies, IgA and IgG by ELISA with Reflex to Immunoblot (2001774). CDC first-line tests for pertussis are PCR (0065080) and/or culture (0060117).
Methodology: Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot
Performed: Tue
Reported: 1-8 days
Specimen Required: Collect: Serum separator tube.

Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Storage/Transport Temperature: Refrigerated.

Unacceptable Conditions: Contaminated, heat-inactivated, or severely lipemic specimens.

Stability (collection to initiation of testing): After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year
Reference Interval:
 
Test Number Components Reference Interval
2001768Bordetella pertussis Antibody, IgG by ELISA with Reflex to Immunoblot  
 
Test Number Components Reference Interval
 Bordetella pertussis Antibody IgG by ELISA 0.9 U/mL or less: Negative - No significant level of detectable Bordetella pertussis IgG antibody.
1.0-2.4 U/mL: Equivocal - Repeat testing in 10-14 days may be helpful.
2.5 U/mL or greater: Positive - IgG antibody to Bordetella pertussis detected, which may indicate a current or past exposure/immunization to B. pertussis.
2004327Bordetella pertussis Antibody, IgG by Immunoblot Refer to report
2001769Bordetella pertussis Antibody, IgM by ELISA with Reflex to Immunoblot  
 
Test Number Components Reference Interval
 Bordetella pertussis Antibody, IgM by ELISA 0.9 U/mL or less: Negative - No significant level of detectable Bordetella pertussis IgM antibody.
1.0-1.1 U/mL: Equivocal - Repeat testing in 10-14 days may be helpful.
1.2 U/mL or greater: Positive - IgM antibody to Bordetella pertussis detected, which may indicate a current or recent exposure/immunization to B. pertussis.
2004326Bordetella pertussis Antibody, IgM by Immunoblot Refer to report
Interpretive Data: Recommend that treatment decisions be based on the result of the B. pertussis IgM immunoblot test instead of the ELISA test.  B. pertussis IgM test by ELISA may produce false-positive results.
Note: If Bordetella pertussis Antibody, IgG by ELISA is 2.5 U/mL or greater, then Bordetella pertussis IgG Immunoblot testing will be added; if Bordetella pertussis Antibody, IgM by ELISA is 1.2 U/mL or greater, then Bordetella pertussis IgM Immunoblot testing will be added. Additional charges apply.
CPT Code(s): 86615 x2; if reflexed, add 86615 for each Immunoblot
Cross References: Pertussis Antibody (Bordetella pertussis Antibodies, IgG & IgM by ELISA with Reflex to Immunoblot) , Whooping Cough (Bordetella pertussis Antibodies, IgG & IgM by ELISA with Reflex to Immunoblot)