ARUP's Laboratory Test Directory

Bordetella pertussis Antibodies, IgA and IgG by ELISA with Reflex to Immunoblot : 2001774

Mnemonic: BORDPAN2

Ordering Recommendation: CDC first-line tests for pertussis are PCR (0065080) and/or culture (0060117). However, if serology is used to assess late-stage pertussis, this is the recommended test.
Methodology: Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot
Performed: Tue
Reported: 1-8 days
Specimen Required: Collect: Serum separator tube.

Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL) Parallel testing is preferred, and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Storage/Transport Temperature: Refrigerated.

Unacceptable Conditions: Contaminated, heat-inactivated, or severely lipemic specimens.

Stability (collection to initiation of testing): After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Reference Interval:
 
Test Number Components Reference Interval
 Bordetella pertussis Antibody, IgA by ELISA with Reflex to Immunoblot  
 
Bordetella pertussis Antibody, IgA by ELISA 0.9 U/mL or less: Negative - No significant level of detectable Bordetella pertussis IgA antibody.
1.0-1.1 U/mL: Equivocal - Repeat testing in 10-14 days may be helpful.
1.2 U/mL or greater: Positive - IgA antibody to Bordetella pertussis detected, which may indicate a current or past exposure/immunization to B. pertussis.
Bordetella pertussis Antibody, IgA by Immunoblot Negative
2001768Bordetella pertussis Antibody, IgG by ELISA with Reflex to Immunoblot  
 
Test Number Components Reference Interval
 Bordetella pertussis Antibody IgG by ELISA 0.9 U/mL or less: Negative - No significant level of detectable Bordetella pertussis IgG antibody.
1.0-2.4 U/mL: Equivocal - Repeat testing in 10-14 days may be helpful.
2.5 U/mL or greater: Positive - IgG antibody to Bordetella pertussis detected, which may indicate a current or past exposure/immunization to B. pertussis.
2004327Bordetella pertussis Antibody, IgG by Immunoblot Refer to report
Interpretive Data: Refer to report.
Note: If Bordetella pertussis Antibody, IgA by ELISA is 1.2 U/mL or greater, then Bordetella pertussis IgA Immunoblot testing will be added; if Bordetella pertussis Antibody, IgG by ELISA is 2.5 U/mL or greater, then Bordetella pertussis IgG Immunoblot testing will be added. Additional charges apply.
CPT Code(s): 86615 x2; if reflexed, add 86615 for each Immunoblot
Cross References: Pertussis Antibody (Bordetella pertussis Antibodies, IgA & IgG by ELISA with Reflex to Immunoblot) , Whooping Cough (Bordetella pertussis Antibodies, IgA & IgG by ELISA with Reflex to Immunoblot)