ARUP's Laboratory Test Directory

Bordetella pertussis Antibody, IgG by ELISA with Reflex to Immunoblot : 2001768

Mnemonic: BORDG

Ordering Recommendation: In most cases, serology testing is not recommended for the diagnosis of active pertussis infection. CDC first-line tests for pertussis are PCR (0065080) and/or culture (0060117).
Methodology: Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot
Performed: Tue
Reported: 1-8 days
Specimen Required: Collect: Serum separator tube.

Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent."

Storage/Transport Temperature: Refrigerated.

Unacceptable Conditions: Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.

Stability (collection to initiation of testing): After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Reference Interval:
 
Test Number Components Reference Interval
 Bordetella pertussis Antibody IgG by ELISA 0.9 U/mL or less: Negative - No significant level of detectable Bordetella pertussis IgG antibody.
1.0-2.4 U/mL: Equivocal - Repeat testing in 10-14 days may be helpful.
2.5 U/mL or greater: Positive - IgG antibody to Bordetella pertussis detected, which may indicate a current or past exposure/immunization to B. pertussis.
2004327Bordetella pertussis Antibody, IgG by Immunoblot  
 
Bordetella pertussis Antibody, IgG by Immunoblot
Effective February 19, 2013
Components Reference Interval
Bordetella pertussis Ab, IgG by Immunoblot Interp Negative
B. pertussis, IgG Immunoblot PT100 Negative
B. pertussis, IgG Immunoblot PT Negative
B. pertussis, IgG Immunoblot FHA Negative

Interpretive Data: Refer to report.
Note: If Bordetella pertussis Antibody, IgG by ELISA is 2.5 U/mL or greater, then Bordetella pertussis IgG Immunoblot testing will be added. Additional charges apply.
CPT Code(s): 86615 Bordetella IgG; if reflexed add 86615 Bordetella IgG Immunoblot
Cross References: Pertussis Antibody (Bordetella pertussis Antibody, IgG by ELISA with Reflex to Immunoblot) , Whooping Cough (Bordetella pertussis Antibody, IgG by ELISA with Reflex to Immunoblot)