| Methodology: |
Cell Culture/Quantitative Enzyme-Linked Immunosorbent Assay
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| Performed: |
Thu
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| Reported: |
1-8 days
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| Specimen Required: |
Collect: At altitudes from sea level to 2,650 feet: Whole blood directly into each of the three tubes in the QuantiFERON®-TB (Standard) Gold In-Tube collection kit (ARUP supply #45112). Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
At altitudes from 2,650-3,350 feet: Collect 3 mL whole blood using a syringe. Transfer 1 mL (Min: 0.8 mL, Max 1.2 mL) to each of the three tubes in QuantiFERON®-TB (Standard) Gold In-Tube collection kit. (ARUP supply #45112).
At altitudes from 3,350-6,150 feet: Whole blood directly into each of the three tubes in the QuantiFERON®-TB (High Altitude)Gold In-Tube collection kit (ARUP supply #46261). Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
At altitudes above 6,150 feet: Collect 3 mL whole blood using a syringe. Transfer 1 mL (Min: 0.8 mL, Max 1.2 mL) to each of the three tubes in QuantiFERON®-TB (High Altitude) Gold In-Tube collection kit.
Tubes should be between 17°C and 25°C (63°F to 77°F) at the time of blood filling.
Specimen Preparation: Immediately after filling tubes, shake them ten (10) times just firmly enough to ensure the entire inner surface of the tube is coated with blood, to solubilize antigens on tube walls. Over-energetic shaking may cause gel disruption and could lead to aberrant results.
After mixing, incubate upright at 37 ° C for 16-24 hours (within 16 hours of collection).
After incubation, centrifuge for 15 minutes at 2000-3000 RCF (g).
Transport plasma in each of the 3 original collection tubes.
Storage/Transport Temperature: Refrigerated.
Remarks: Further information on mixing QuantiFERON-TB Gold In-Tube collection tubes and an instructional video can be found at: http://www.aruplab.com/Specimen-Handling/SpecialSpecimenCollection/quantiferon.jsp
Unacceptable Conditions: Room temperature specimens. Specimens that have not been centrifuged.
Stability (collection to initiation of testing): After incubation and centrifugation: Ambient: 2 hours; Refrigerated: 4 weeks; Frozen: 4 months
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