ARUP's Laboratory Test Directory
| 0098268: Hepatitis C Virus RNA Quantitative, Real-Time PCR |
| Test Mnemonic: HEPCQNT | |
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Methodology: Real-Time Polymerase Chain Reaction
Performed: Sun-Sat Reported: 1-3 days |
| Specimen Required: | |
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Collect: One 6 mL SST, PPT, or one 5 mL lavender (EDTA) or pink (K2EDTA).
Transport: 2 mL serum or plasma, frozen. (Min: 0.5 mL) Submit specimen in an ARUP Standard Transport Tube. Specimens collected in a PPT should be submitted in original container. Submit specimen according to Biological Substance, Category B, shipping guidelines. Pediatric Collection/Transport: 0.25 mL serum or plasma, frozen. Submit specimen according to Biological Substance, Category B, shipping guidelines. Remarks: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered. Separate serum or plasma from cells within two hours. Unacceptable Conditions: Nonfrozen, heparinized, thawed, or refrozen specimens. Polystyrene tubes. Stability: Ambient: 2 hours (on cells); Plasma or serum removed from cells within two hours can be refrigerated and frozen as follows: Refrigerated: 12 hours; Frozen: 4 months |
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| Reference Interval: |
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Less than 75 IU/mL Less than log 1.9 IU/mL |
| Interpretive Data: | |
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Assay methodology is polymerase chain reaction (PCR) using the ROCHE COBAS TaqMan® Analyte Specific Reagent. Results below the limit of detection (1.9 log IU/mL) will be reported as <1.9 log IU/mL. Results between 1.9 and 2.3 log IU/mL (75 to 200 IU/mL) will be reported as "HCV RNA detected below the limit of quantitation." Results 2.3 log IU/mL (200 IU/mL) and greater will be reported with a numeric value. False positive results may occur. A result of less than 75 HCV IU/mL does not rule out the presence of PCR inhibitors in the patient specimen or hepatitis C virus RNA concentrations below the level of detection by the assay. This assay should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular Tissue-Based Products (HCT/P). Please refer to Statement A in the Compliance Statements section in the front of the Laboratory Test Directory. |
| Note: | |
| A qualitative PCR assay with a detection limit of 50 HCV RNA IU/mL is available for the assessment of lower levels of HCV viremia, (refer to test Hepatitis C Virus RNA Qualitative PCR 0098264). |
| CPT Code(s): | |
| 87522 |