ARUP's Laboratory Test Directory

Hepatitis C Virus RNA Qualitative PCR : 0098264

Mnemonic: HEP C PCR

Methodology: Polymerase Chain Reaction
Performed: Sun-Sat
Reported: 2-4 days
Specimen Required: Collect:  One 6 mL serum separator tube, PPT, or one 5 mL lavender (EDTA) or pink (K2EDTA).

Transport:  2 mL serum or plasma, frozen. (Min: 0.5 mL) Submit specimen in an ARUP Standard Transport Tube.  Specimens collected in a PPT should be submitted in original container.

Submit specimen according to Biological Substance, Category B, shipping guidelines.


Pediatric Collection/Transport:  0.25 mL serum or plasma, frozen. 

Submit specimen according to Biological Substance, Category B, shipping guidelines.


Remarks:  CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered. Separate serum or plasma from cells within two hours.

Unacceptable Conditions:  Specimens collected in ACD.  Nonfrozen or heparinized specimens. Specimens thawed and refrozen. Polystyrene tubes.

Stability:  Ambient: 2 hours (on cells);  Plasma or serum removed from cells within two hours can be refrigerated and frozen as follows: Refrigerated: 12 hours; Frozen: 4 months
Reference Interval:
Negative
Interpretive Data: The lower limit of detection is 50 IU/mL.

Assay methodology is polymerase chain reaction (PCR) using the FDA approved Roche COBAS Amplicor Hepatitis C Virus test, Version 2.0.

An internal control monitors the specimen for inadequate specimen extraction or the presence of inhibitors in the specimen. False positive results may occur.  A negative result does not rule out the presence of PCR inhibitors in the patient specimen or hepatitis C virus RNA concentrations below the limit of detection by the assay.

This assay should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular Tissue-Based Products (HCT/P).

CPT Code(s): 87521 
 
 

 

 

 
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