Reference Interval:
| Components
| Reference Interval
| | % Normal PMNs
| By report
| | % PNH Cells
| By report
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| Interpretive Data: |
The High Resolution PNH-WBC panel detects the absence of phosphatidylinositol-linked membrane proteins CD55 (DAF-decay accelerating factor) and CD59 (MIRL-membrane inhibitor of reactive lysis) on neutrophils (PMNs). The PMNs are identified with CD11b staining. All normal PMNs (99.997-100.000%) express CD55 and CD59 and are reported as % Normal PMNs. The expression of CD55 and CD59 is decreased or absent in a subpopulation of PMNs in patients with paroxysmal nocturnal hemoglobinuria. The estimated percentage of PMNs with decreased expression is reported as % PNH cells. Since 'diagnosis of PNH disease requires a deficiency of at least two GPI-linked antigens in two different cell lineages'*, this test may be complemented with a PNH test (0095864), if clinically indicated. *Cytometry 42:223-33 (2000)
The detection of a very low percentage of PNH cells (0.004-1.000%) may be found in patients with aplastic anemia, myelodysplasia, refractory anemia, refractory anemia with excess blasts, and myelofibrosis. The presence of PNH cells in these patients may correlate with a better prognosis. Blood 102:1211-1218 (2003)
Please refer to Statement A in the Compliance Statements section in the front of the Laboratory Test Directory.
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| CPT Code(s): |
88184 First marker; 88185 x2 Each additional marker
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Cross References: |
PI-linked Antigen on WBCs
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