ARUP's Laboratory Test Directory
| 0096657: Neutrophil Oxidative Burst Assay (DHR) |
![[ image for: Time Sensitive]](icon_1.jpg){kind=icon} | Time Sensitive |
| Test Mnemonic: DHR | |
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Methodology: Flow Cytometry
Performed: Sun-Sat Reported: 2-3 days |
| Specimen Required: | |
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Collect: One 3 mL green (sodium heparin) and one normal control in a 3 mL green (sodium heparin) from a healthy unrelated individual.
Transport: 3 mL whole blood and 3 mL normal control in sterile containers at 20-25°C. LIVE NEUTROPHILS REQUIRED. CRITICAL AMBIENT. Do not refrigerate or freeze. Pediatric Collection/Transport: 3 mL whole blood (green, sodium heparin) and 3 mL whole blood (green, sodium heparin) normal control from a healthy unrelated individual in sterile containers at 20-25°C. Remarks: Patient and control samples must be collected within 48 hours of test performance. Unacceptable Conditions: Refrigerated or frozen samples or samples in transport longer than 48 hours. Stability: Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable |
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| Reference Interval: |
| By report |
| Interpretive Data: | |
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White blood cells are incubated with dihydrorhodamine 123 (DHR) and catalase, then stimulated with Phorbol 12-Myristate 13-Acetate (PMA). Dihydrorhodamine oxidation to rhodamine by the respiratory burst of the cell is measured by flow cytometry. Results are reported as the ratio of the mean channel fluorescence of stimulated cells versus unstimulated cells, which yields a stimulation index (SI). Please refer to Statement B in the Compliance Statements section in the front of the Laboratory Test Directory. |
| Note: | |
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If sample shows abnormal results when stimulated, and no control was sent, test should be resubmitted with control sample to validate the conditions of collection, processing and transport. For abnormal results, we encourage consultation with the ARUP Immunology Medical Director. Interpretation comparing the patient results to the client normal control and the laboratory control will be provided by the medical director. |
| CPT Code(s): | |
| 88184 First marker; 88185 Additional marker; 88187 Interpretation 2-8 markers |