ARUP's Laboratory Test Directory

Neutrophil Oxidative Burst Assay (DHR) : 0096657
[ image for: Time Sensitive]
Time Sensitive
  


Mnemonic: DHR

Methodology: Semi-Quantitative Flow Cytometry
Performed: Sun-Sat
Reported: 2-3 days
Specimen Required: Patient Prep: Collect control specimen from a healthy individual unrelated to patient at approximately the same time as and under similar conditions to the patient.

Collect: Green (sodium or lithium heparin) (patient) AND green (sodium or lithium heparin) (control). Patient and control specimens must be collected within 48 hours of test performance.

Specimen Preparation: Transport 3 mL whole blood (patient) AND 3 mL whole blood (control) in original collection tubes. Do not refrigerate or freeze. LIVE NEUTROPHILS REQUIRED.

Storage/Transport Temperature: CRITICAL ROOM TEMPERATURE.

Remarks:

Unacceptable Conditions: Refrigerated or frozen specimens. Specimens in transport longer than 48 hours.

Stability (collection to initiation of testing): Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable

Reference Interval:
By report
Interpretive Data: White blood cells are incubated with dihydrorhodamine 123 (DHR) and catalase, then stimulated with Phorbol 12-Myristate 13-Acetate (PMA). Dihydrorhodamine oxidation to rhodamine by the respiratory burst of the cell is measured by flow cytometry.

Results are reported as the ratio of the mean channel fluorescence of stimulated cells versus unstimulated cells, which yields a stimulation index (SI).

See Compliance Statement B: www.aruplab.com/CS
Note: If sample shows abnormal results when stimulated, and no control was sent, test should be resubmitted with control sample to validate the conditions of collection, processing and transport.  For abnormal results, we encourage consultation with the ARUP Immunology Medical Director.

Interpretation comparing the patient results to the client normal control and the laboratory control will be provided by the medical director.
CPT Code(s): 86352