ARUP's Laboratory Test Directory

0096299: Leukemia/Lymphoma Phenotyping (Comprehensive-Whole Blood)

[ image for: Flow Cytometry Test Request Form Recommended]
Flow Cytometry Test Request Form Recommended
  

Test Mnemonic: LEUK-WB
Methodology: Flow Cytometry

Performed: Sun-Sat

Reported: Within 3 days

Specimen Required:  
Collect: One 5 mL green (sodium heparin), lavender (EDTA), or pink (K2EDTA).

Transport: 5 mL whole blood at 20-25°C.

Remarks: Sample should be received within 24 hours of collection for optimal viable testing.  Please provide CBC, Wright's stained smear, clinical history, differential diagnosis, and any relevant pathology reports. Refrigerated samples are also acceptable.

Unacceptable Conditions: Clotted, hemolyzed, or frozen samples.  Samples greater than 48 hours old.

Stability: Ambient: 48 hours; Refrigerated: 48 hours; Frozen Unacceptable

Reference Interval:
By report

Available Antigens Included
:
T-cell:
CD1, CD2, CD3, CD4, CD5, CD7, CD8, TCR alpha-beta, TCR gamma-delta, Cytoplasmic CD3
B-cell:
CD10, CD19, CD20, CD22, CD23, CD24, CD79b, CD103, Kappa, Lambda, FMC7, Cytoplasmic Kappa, Cytoplasmic Lambda
Myelo/Mono:
CD11b, CD13, CD14 (Mo2), CD14 (MY4), CD15, CD33, CD64, CD117, myeloperoxidase
Misc:
CD11c, CD16, CD25, CD30, CD34, CD38, CD41, CD42b, CD45, CD56, CD57, CD61, HLA-DR, glycophorin, TdT, bcl-2

Interpretive Data:


Please refer to Statement A in the Compliance Statements section in the front of the Laboratory Test Directory.



Note:
Surface markers in leukemia and lymphoma may aid in identifying the tumor lineage for diagnostic and prognostic purposes. After review of the clinical history and morphology, a panel of markers is selected for each case by a board-certified pathologist. In most cases, the lineage can be identified as T-cell, B-cell, or myeloid and a diagnosis, or differential diagnosis, can be made.

CPT Code(s):
88184 First marker and 88185 Each additional marker; AND 88187 Interpretation for 2-8 markers or 88188 Interpretation for 9-15 markers or 88189 Interpretation for 16 or more markers.

 

 

 
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