ARUP's Laboratory Test Directory

Lymphocyte Antigen Proliferation : 0096055
[ image for: Time Sensitive]
Time Sensitive
  


Mnemonic: LAPP

Methodology: Cell Culture
Performed: Tue-Fri
Reported: 9-10 days
Specimen Required: Patient Prep: Collect control specimen from a healthy individual unrelated to patient at approximately the same time as and under similar conditions to the patient.

Collect: Green (sodium heparin) (patient) AND green (sodium heparin) (control). Also acceptable: Yellow (ACD Solution A) (patient) AND yellow (ACD Solution A) (control). Patient and control specimens must be collected within 48 hours of test performance.

Specimen Preparation: Transport 10 mL whole blood (patient) AND 10 mL whole blood (control) in original collection tubes. (Min: 7 mL (patient) AND 7 mL (control)) Do not refrigerate or freeze. LIVE LYMPHOCYTES REQUIRED.
Infant Minimum: 3 mL (patient) AND 7 mL (control).

Storage/Transport Temperature: CRITICAL ROOM TEMPERATURE.

Remarks:

Unacceptable Conditions: Yellow (ACD Solution B). Refrigerated or frozen specimens. Specimens in transport longer than 48 hours.

Stability (collection to initiation of testing): Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Reference Interval:
By report
Interpretive Data: Candida and tetanus antigens are tested independently in lymphocyte culture. Lymphocyte proliferation in response to these antigens is determined by 3H-thymidine incorporation.

Results are reported as the counts per minute (CPM) antigen stimulated versus control cultures and a stimulation index (SI) which represents the ratio of mean CPM of the stimulated lymphocytes to the mean CPM of the unstimulated control.

See Compliance Statement B: www.aruplab.com/CS
Note: Interpretation comparing the patient results to the client normal control and the laboratory control will be provided by the medical director.
CPT Code(s): 86353 x2
Cross References: Antigen Proliferation, Lymphocytes (Lymphocyte Antigen Proliferation)