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Lymphocyte Mitogen Proliferation
Ordering Recommendation
Primarily used for research and to support attempts to understand the pathogenesis of immune, infectious, allergic, or inflammatory disorders. For patients who are lymphopenic, collection of 10 mL of whole blood, rather than the minimum required volumes, is recommended to increase the likelihood that a sufficient number of lymphocytes can be isolated for testing.
New York DOH Approval Status
Specimen Required
Collect control specimen from a healthy individual unrelated to patient.
Green (sodium heparin) (patient) AND green (sodium heparin) (control). Also acceptable: Yellow (ACD solution A) (patient) AND yellow (ACD solution A) (control). Patient and control specimens must be collected within 48 hours of test performance.
Transport 10 mL whole blood (patient) AND 10 mL whole blood (control) in original collection tubes. (Min: 7 mL (patient) AND 7 mL (control)) Do not refrigerate or freeze. LIVE LYMPHOCYTES REQUIRED.
Infant Minimum: 3 mL (patient) AND 7 mL (control).
For patients who are lymphopenic, collection of 10 mL of whole blood, rather than the minimum required volumes, is recommended to increase the likelihood that a sufficient number of lymphocytes can be isolated for testing.
CRITICAL ROOM TEMPERATURE.
Refrigerated or frozen specimens. Specimens in transport longer than 48 hours.
Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
New York State Clients: Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Methodology
Cell Culture
Performed
Sun, Tue, Wed, Thu, Fri, Sat
Reported
9-10 days
Reference Interval
By report
Interpretive Data
Lymphocyte proliferation in response to the nonspecific mitogens phytohemagglutinin (PHA), concanavalin A (Con A), and pokeweed are determined by 3H -thymidine incorporation.
Results are reported as counts per minute (CPM) mitogen stimulated versus a control culture and a stimulation index (SI) which represents the ratio of CPM of the stimulated lymphocytes to the mean CPM of the unstimulated control.
Laboratory Developed Test (LDT)
Note
Interpretation comparing the patient results to the client normal control and the laboratory control will be provided by the medical director.
Hotline History
CPT Codes
86353 x3
Components
| Component Test Code* | Component Chart Name | LOINC |
|---|---|---|
| 0096044 | Lymphocyte Mitogen Proliferation | 53810-8 |
Aliases
- Mitogen Proliferation, Lymphocytes
