Reference Interval:
Reports include age appropriate reference intervals and interpretation.
| Components
| Reference Interval
| % CD4 (Helper T-cells)
Absolute CD4
| By report
| % CD45RA (Naive helper T-cells)
Absolute CD45RA
| By report
| % CD45RO (Memory helper T-cells)
Absolute CD45RO
| By report
| % CD8 (Suppressor T-cells)
Absolute CD8
| By report
| | CD4: CD8 ratio
| By report
| % CD3 (Total T-cells)
Absolute CD3
| By report
| % CD19 (B-cells)
Absolute CD19
| By report
| % NK-cells
Absolute NK-cells
| By report
|
|
| Interpretive Data: |
In this test, the CD4 cells are Helper T-cells because they express both CD3 and CD4. The CD8 cells are Suppressor T-cells because they express both CD3 and CD8. The B-cells express CD19 but not CD3. The NK-cells express either CD16 or CD56 (or both) but not CD3. The CD45RA cells express both CD4 and CD45RA and the CD45RO cells express both CD4 and CD45RO. Helper T-cell levels are a criterion for categorizing HIV-related clinical conditions by the CDC's classification system for HIV infection. The measurement of Helper T-cell levels has been used to establish decision points for initiating P. jiroveci prophylaxis and antiviral therapy and to monitor the efficacy of treatment. The Public Health Service (PHS) has recommended that Helper T-cell levels be monitored every three to six months in all HIV-infected persons.
The performance characteristics of this test were determined by ARUP Laboratories, Inc.
See Compliance Statement A: www.aruplab.com/CS
|
| Note: |
This assay is designed for enumerating the percents and absolute cell counts of lymphocyte subsets in lysed whole blood. Whole blood is added to fluorochrome-labeled antibodies that bind specifically to cell surface antigens on lymphocytes. After incubation, lysing and fixation, percents and absolute counts are enumerated utilizing an internal quantitation standard. Additional CBC data is not required.
During HIV infection, antiviral therapy is often initiated when the absolute CD4 count drops below 500 cells/µL. When the absolute CD4 count drops below 200 cells/µL, therapeutic prophylaxis against PCP and other opportunistic infections may be initiated. When the absolute CD4 count drops below 100 cells/µL, prophylaxis against Mycobacterium avium complex is recommended.
The CD45RA subset delineates a "naive" helper T-cell population. The CD45RO subset delineates a "memory" helper T-cell population. With disease progression in HIV-infected individuals, a decrease in CD45RA "naive" cells may be observed.
For longitudinal studies involving serial monitoring, specimen collections should be performed at the same time of day.
|
| CPT Code(s): |
86355; 86357; 86359; 86360; 86356 x3
|
Cross References: |
T-Cell Immunodeficiency Extended Profile (Lymphocyte Subset Panel 6 - Total Lymphocyte Enumeration with CD45RA & CD45RO)
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