ARUP's Laboratory Test Directory

Lymphocyte Transplantation Profile : 0095798
[ image for: Time Sensitive]
Time Sensitive
  


Mnemonic: LYMPHPRO

Methodology: Flow Cytometry
Performed: Sun-Sat
Reported: Within 24 hours
Specimen Required: Collect:  One 5 mL green (sodium or lithium heparin), lavender (EDTA), or pink (K2EDTA).
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Transport:  5 mL whole blood at 20-25°C. (Min: 1 mL)

Pediatric Collection/Transport:  0.75 mL whole blood at 20-25°C.

Remarks:  CRITICAL AMBIENT.  THIS TEST IS FOR TRANSPLANT PATIENTS ONLY. WBC and lymph % results with the same draw date and time as the CD3 sample must be provided by client. Specimens must be analyzed within 48 hours of collection. Some medications may affect immunophenotyping results and should be listed on the patient test request form.
New York State Clients:
EDTA specimens must be analyzed within 30 hours of collection.  Heparin specimens must be analyzed within 48 hours of collection.


Unacceptable Conditions:  Clotted, refrigerated, hemolyzed, or frozen specimens. Specimens older than 48 hours.
New York State Clients:
Clotted, refrigerated, hemolyzed, or frozen specimens.  EDTA specimens older than 30 hours and heparin specimens older than 48 hours.


Stability:  Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
New York State Clients:
Ambient: EDTA specimens - 30 hours, Heparin specimens - 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable

Reference Interval:
 
Components 0-11 months 12-23 months 2 years and older
%CD2 55-88 55-88 74-92
Absolute CD2 3800-5300 3100-4200 560-2600
%CD3 58-85 53-81 60-83
Abs CD3 2170-6500 1460-5440 450-2300
%CD4 38-62 31-54 32-63
%CD8 16-34 16-38 14-41
CD4: CD8 ratio 1.17-6.62 1.17-6.62 0.8-5.0
%CD19 11-45 11-45 5-23

Interpretive Data: In this test, the percent CD3 cells are those cells that are positive for both CD3 and the alpha-beta chain of the T-cell receptor and are presumed immunologically competent.

Please refer to Statement A in the Compliance Statements section in the front of the Laboratory Test Directory.
Note: THIS TEST IS FOR TRANSPLANT PATIENTS ONLY.  Immunosuppressive therapy with anti-lymphocyte drugs, such as OKT3 or ATG (anti-thymocyte globulin), can be monitored with this test.  For immunocompromised patients, order Lymphocyte Subset Panel 5 - Total Lymphocyte Enumeration (0095892).
CPT Code(s): 88184 First marker; 88185 x5 Each additional marker
 
 

 

 

 
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