ARUP's Laboratory Test Directory
| 0095735: DNA Content/Cell Cycle Analysis, Breast (Paraffin) |
| Test Mnemonic: DNA HIST P | |
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Methodology: Flow Cytometry
Performed: Sun, Tue Reported: 3-9 days |
| Specimen Required: | |
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Collect: Breast tumor tissue.
Transport: Paraffin-embedded tissue block enriched with breast tumor at 20-25°C. Remarks: Please include H & E stained slide and surgical pathology report. If no normal tissue is included in the block, please supply a control tissue block to be processed in parallel. Unacceptable Conditions: No tumor tissue remaining on block. Samples fixed in Bouin's solution (picric acid), mercuric chloride containing fixatives (e.g., B5, Zenker's solution) or ethanol-based fixatives containing ethylene glycol, acetic acid, and zinc chloride. Samples that have been decalcified. Stability: Paraffin block: Ambient: Indefinitely; Refrigerated: Acceptable; Frozen: Unacceptable |
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| Reference Interval: |
| Report components include: DNA content, S-phase interpretation, and a copy of the histogram. |
| Interpretive Data: | |
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In breast cancer, many studies indicate that low S-phase and diploid DNA content are associated with a relatively good prognosis. Conversely, the presence of aneuploid DNA content and a high S-phase tends to correlate with a worse prognosis.
Please refer to Statement B in the Compliance Statements section in the front of the Laboratory Test Directory. |
| Note: | |
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A thin section of each tissue submitted is stained with H & E to verify the presence of tumor. The DNA content of each tumor is classified, in order of worsening prognosis, as diploid, near diploid, tetraploid, aneuploid, or hypertetraploid. The DNA index is the ratio of tumor G0-G1 cells to normal G0-G1 cells. The tumor-specific S-phase is used when possible. An average histogram S-phase is used for diploid and some aneuploid tumors where the tumor and host S-phases cannot be separated. An average histogram S-phase is also used when the percentage of aneuploid cells in the histogram is low (less than 25%). The S-phase interpretation is based on comparison to over 5,000 breast tumors, controlled for histogram type (diploid, near-diploid, aneuploid) and sample type (fresh/frozen, paraffin). The appropriate population is divided into thirds and compared to the sample S-phase for an interpretation of low, moderate, or high S-phase. |
| CPT Code(s): | |
| 88182 |