ARUP's Laboratory Test Directory
| 0095614: Platelet Associated Antibodies, Direct Assay |
| Test Mnemonic: PAIG | |
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Methodology: Flow Cytometry
Performed: Sun-Sat Reported: 2-3 days |
| Specimen Required: | |
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Collect: Two 5 mL lavender (EDTA) or pink (K2EDTA).
Transport: 10 mL whole blood at 20-25°C. (Min: 5 mL) Pediatric Collection/Transport: 5 mL whole blood at 20-25°C. Remarks: CRITICAL AMBIENT. Samples must be received within 48 hours of collection. Required amount of blood may be dependent on platelet count. Unacceptable Conditions: Clotted, hemolyzed, refrigerated, or frozen samples. Samples greater than 48 hours old. Stability: Ambient: 2 days; Refrigerated: Unacceptable; Frozen: Unacceptable |
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| Reference Interval: |
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IgG: Negative IgM: Negative |
| Interpretive Data: | |
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Negative (IgG & IgM): No excess antibodies were associated with the patient's platelets. An immune cause of thrombocytopenia is unlikely. Positive (IgG and/or IgM): An increase in platelet associated immunoglobulin is noted. An immune cause of thrombocytopenia should be considered. However, many conditions can result in an increase in platelet associated antibodies; for example, IgM rheumatoid factor antibodies. Strong Positive (IgG and/or IgM): A definite increase in platelet associated immunoglobulin is noted and an immune cause of thrombocytopenia should be considered. However, many conditions can result in an increase in platelet associated antibodies; for example, IgM rheumatoid factor antibodies. Please refer to Statement A in the Compliance Statements section in the front of the Laboratory Test Directory. |
| Note: | |
| Detection of platelet-associated IgG and/or IgM may be used to separate thrombocytopenia of immune origin from nonimmune origin. Most patients with ITP have abnormally high levels of IgG associated with their platelets. Occasionally patients will have normal IgG levels but abnormally high levels of IgM. Dual staining and flow cytometric analysis ensures that only platelets are analyzed and relatively small volumes of blood are required. This assay does not detect platelet specific alloantibodies. Refer to Platelet Antibodies, Indirect (0051050). |
| CPT Code(s): | |
| 86023 IgG; 86023 IgM |