ARUP's Laboratory Test Directory
| 0095243: Leukemia/Lymphoma Phenotyping (Comprehensive - Miscellaneous) |
![[ image for: Flow Cytometry Test Request Form Recommended]](icon_12.jpg){kind=icon} | Flow Cytometry Test Request Form Recommended |
| Test Mnemonic: LEUK MS | |
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Methodology: Flow Cytometry
Performed: Sun-Sat Reported: Within 3 days |
| Specimen Required: | |
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Collect: Tissue or fluid.
Transport: 100 mg fresh tissue suspended in tissue culture media (e.g., RPMI 1640) on refrigerant pack or 10-100 mL fresh fluid on refrigerant pack. (Min: 100 mg tissue or 10 mL fluid) Pediatric Collection/Transport: 100 mg tissue suspended in tissue culture media (e.g., RPMI 1640) on refrigerant pack or 10-100 mL fresh fluid on refrigerant pack. Remarks: Sample must be received within 24 hours of collection for optimal viable testing. Please provide sample source, clinical history, differential diagnosis, and any relevant pathology reports. In general, a minimum of 10,000 viable cells is required for flow cytometry phenotyping with a very limited number of antibodies. All specimens, regardless of quality, will be processed and analyzed. Supplying clinical and diagnostic information is especially important with low count specimens to help ensure the most appropriate antibody combinations are evaluated before the sample is depleted of cells. Unacceptable Conditions: Clotted or frozen samples. Samples greater than 48 hours old. Stability: Refrigerated: 48 hours; Frozen: Unacceptable |
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| Reference Interval: |
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By report Available Antigens Included: T-cell: CD1, CD2, CD3, CD4, CD5, CD7, CD8, TCR alpha-beta, TCR gamma-delta, Cytoplasmic CD3 B-cell: CD10, CD19, CD20, CD22, CD23, CD24, CD79b, CD103, Kappa, Lambda, FMC7, Cytoplasmic Kappa, Cytoplasmic Lambda Myelo/Mono: CD11b, CD13, CD14 (Mo2), CD14 (MY4), CD15, CD33, CD64, CD117, myeloperoxidase Misc: CD11c, CD16, CD25, CD30, CD34, CD38, CD41, CD42b, CD45, CD56, CD57, CD61, HLA-DR, glycophorin, TdT, bcl-2 |
| Interpretive Data: | |
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Please refer to Statement A in the Compliance Statements section in the front of the Laboratory Test Directory. |
| Note: | |
| Surface markers in leukemia and lymphoma may aid in identifying the tumor lineage for diagnostic and prognostic purposes. After review of the clinical history and morphology, a panel of markers is selected for each case by a board-certified pathologist. In most cases, the lineage can be identified as T-cell, B-cell, or myeloid and a diagnosis, or differential diagnosis, can be made. |
| CPT Code(s): | |
| 88184 First marker and 88185 Each additional marker; AND 88187 Interpretation for 2-8 markers or 88188 Interpretation for 9-15 markers or 88189 Interpretation for 16 or more markers. |