ARUP's Laboratory Test Directory

Leukemia/Lymphoma Phenotyping (Comprehensive - Miscellaneous) : 0095243
[ image for: Flow Cytometry Test Request Form Recommended]
Flow Cytometry Test Request Form Recommended
  


Mnemonic: LEUK MS

Methodology: Flow Cytometry
Performed: Sun-Sat
Reported: 1-2 days
Specimen Required: Collect: Tissue or fluid.

Specimen Preparation: Tissue: Suspend 100 mg fresh tissue in tissue culture media (e.g., RPMI 1640). (Min: 100 mg)
Fluid:
Transport 10-100 mL fresh fluid. (Min: 10 mL)


Storage/Transport Temperature: Refrigerated on refrigerant pack. Specimen must be received within 24 hours of collection for optimal viable testing.

Remarks: Provide specimen source, clinical history, differential diagnosis, and any relevant pathology reports. In general, a minimum of 10,000 viable cells is required for flow cytometry phenotyping with a very limited number of antibodies. All specimens, regardless of quality, will be processed and analyzed. Supplying clinical and diagnostic information is especially important with low count specimens to help ensure the most appropriate antibody combinations are evaluated before the specimen is depleted of cells.

Unacceptable Conditions: Frozen specimens. Clotted specimens. Specimens greater than 48 hours old.

Stability (collection to initiation of testing): Ambient: Unacceptable; Refrigerated: 48 hours; Frozen: Unacceptable
Reference Interval:
By report

Available Antigens Included:

*Not all markers will be reported in all cases. Requests for specific markers to be included must be listed on manual requisition or by footnote for electronic orders. We do not offer individual markers without also running our panel.

T-cell: CD1, CD2, CD3, CD4, CD5, CD7, CD8, TCR alpha-beta, TCR gamma-delta, Cytoplasmic CD3
B-cell: CD10, CD19, CD20, CD22, CD23, CD103, Kappa, Lambda, FMC7, Cytoplasmic Kappa, Cytoplasmic Lambda
Myelo/Mono: CD11b, CD13, CD14 (Mo2), CD14 (MY4), CD15, CD33, CD64, CD117, myeloperoxidase
Misc: CD11c, CD16, CD25, CD30, CD34, CD38, CD41, CD42b, CD45, CD56, CD57, CD61, HLA-DR, glycophorin, TdT, bcl-2
Interpretive Data:

Refer to Statement A under Testing Information at http://www.aruplab.com.
Note: Surface markers in leukemia and lymphoma may aid in identifying the tumor lineage for diagnostic and prognostic purposes. After review of the clinical history and morphology, a panel of markers is selected for each case by a board-certified pathologist. In most cases, the lineage can be identified as T-cell, B-cell, or myeloid and a diagnosis, or differential diagnosis, can be made.
CPT Code(s): 88184 First marker and 88185 Each additional marker; AND 88187 Interpretation for 2-8 markers or 88188 Interpretation for 9-15 markers or 88189 Interpretation for 16 or more markers.
 
 

 

 

 
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