Reference Interval:
Report components include: DNA content, S-phase interpretation, and copy of histogram.
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| Interpretive Data: |
Stage A and B prostate carcinomas are approximately 70% diploid and 30% nondiploid. Stage C and D carcinomas are approximately 40% diploid, 50% tetraploid and 10% aneuploid. About 15% of men with diploid prostate carcinoma and 50% of men with nondiploid carcinoma, will show five-year disease progression. The four-year death rate for men with diploid, tetraploid, aneuploid, and multiple aneuploid carcinomas is approximately 5%, 30%, 60%, and 75%, respectively. DNA analysis shows no prognostic significance in Stage D2 carcinomas.
Refer to Statement B under Testing Information at http://www.aruplab.com.
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| Note: |
A thin section of each tissue submitted is stained with H & E to verify the presence of tumor. The DNA content of each tumor is classified, in order of worsening prognosis, as diploid, tetraploid, aneuploid, or multiple aneuploid. The DNA index is the ratio of tumor G 0-G1 cells to normal G0-G1\f1 cells.
The tumor-specific S-phase is used when possible. An average histogram S-phase is used for diploid and aneuploid tumors where the tumor and host S-phases cannot be separated. An average histogram S-phase is also used when the percentage of aneuploid cells in the histogram is low (less than 25%). The S-phase interpretation is based on comparison to other prostate tumors and is controlled for histogram type (diploid, aneuploid). The appropriate population is divided into thirds and compared to the sample S-phase for an interpretation of low, moderate, or high S-phase.
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| CPT Code(s): |
88182
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Cross References: |
DNA Ploidy and S-Phase (Paraffin Block) (DNA Content/Cell Cycle Analysis, Prostate) |
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