ARUP's Laboratory Test Directory
| 0093420: Lymphocyte Subsets Panel 4 - T-Cell Subsets Percents & Ratio, Bronchoalveolar Lavage |
| Time Sensitive |   |   |
| Test Mnemonic: LYMBAL | |
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#ExistMethodology>
Methodology: Flow Cytometry
*ExistMethodology> #ExistPerformed> Performed: Sun-Sat *ExistPerformed> #ExistReported> Reported: 1-3 days *ExistReported> |
| Specimen Required: | |
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#ExistCollect>
Collect: Bronchoalveolar lavage.
*ExistCollect> #ExistTransport> Transport: 5 mL bronchoalveolar lavage in a sterile container at 2-8°C. Submit specimen according to Biological Substance, Category B, shipping guidelines. *ExistTransport> #ExistPedCollectTransport> Pediatric Collection/Transport: 3 mL bronchoalveolar lavage at 2-8°C. Submit specimen according to Biological Substance, Category B, shipping guidelines. *ExistPedCollectTransport> #ExistRemarks> Remarks: Specimens must be analyzed within 48 hours of collection. *ExistRemarks> #ExistConditions> Unacceptable Conditions: Ambient, frozen, contaminated, excess mucus, too few lymphocytes, and specimens greater than 48 hours old. Whole blood specimens (refer to 0095950). *ExistConditions> #ExistStability> Stability: Ambient: Unacceptable, Refrigerated: 48 hours, Frozen: Unacceptable *ExistStability> |
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| Reference Interval: |
| #ExistRefRange> By report. *ExistRefRange> |
| Interpretive Data: | |
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#ExistInterpData>
Sarcoidosis is frequently associated with a predominance of CD4 positive T-Cells. This causes an increase in the CD4/CD8 ratio. If the CD4/CD8 ratio is >5.0 and clinical and radiographic findings are compatible with sarcoidosis, some institutions accept a diagnosis of sarcoidosis even if the biopsies are negative. In contrast to active sarcoidosis, in extrinisic allergic alveolitis (EAA), there is a relative increase in the CD8 positive T-Cells resulting in a decrease in the CD4/CD8 ratio. Eur Respir J, 24:1000-6; 2004. The performance characteristics of this test were determined by ARUP Laboratories, Inc. *ExistInterpData> |
| Note: | |
| This assay is designed for enumerating the percents of T-Cell lymphocyte subsets in BAL and other body fluid samples (CSF, Pleural, Peritoneal). These fluids are added to fluorochrome-labeled antibodies that bind specifically to cell surface antigens on lymphocytes. The CD4/CD8 ratio is calculated by dividing the CD4% by the CD8%. |
| CPT Code(s): | |
| 88184 CD3; 88185 CD4; 88185 CD8 |