Lymphocyte Subsets Panel 4 - T-Cell Subsets Percents & Ratio, Bronchoalveolar Lavage : ARUP Lab Tests

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Lymphocyte Subsets Panel 4 - T-Cell Subsets Percents & Ratio, Bronchoalveolar Lavage : 0093420

Time Sensitive

Mnemonic: LYMBAL

Methodology:	Flow Cytometry
Performed:	Sun-Sat
Reported:	1-3 days
Specimen Required:	Collect: Bronchoalveolar lavage. Transport: 5 mL bronchoalveolar lavage in a sterile container at 2-8°C. Submit specimen according to Biological Substance, Category B, shipping guidelines. Pediatric Collection/Transport: 3 mL bronchoalveolar lavage at 2-8°C. Submit specimen according to Biological Substance, Category B, shipping guidelines. Remarks: Specimens must be analyzed within 48 hours of collection. Unacceptable Conditions: Ambient, frozen, contaminated, excess mucus, too few lymphocytes, and specimens greater than 48 hours old. Whole blood specimens (refer to 0095950). Stability: Ambient: Unacceptable, Refrigerated: 48 hours, Frozen: Unacceptable

Reference Interval: By report.
Interpretive Data:	Sarcoidosis is frequently associated with a predominance of CD4 positive T-Cells. This causes an increase in the CD4/CD8 ratio. If the CD4/CD8 ratio is >5.0 and clinical and radiographic findings are compatible with sarcoidosis, some institutions accept a diagnosis of sarcoidosis even if the biopsies are negative. In contrast to active sarcoidosis, in extrinisic allergic alveolitis (EAA), there is a relative increase in the CD8 positive T-Cells resulting in a decrease in the CD4/CD8 ratio. Eur Respir J, 24:1000-6; 2004. The performance characteristics of this test were determined by ARUP Laboratories, Inc.
Note:	This assay is designed for enumerating the percents of T-Cell lymphocyte subsets in BAL and other body fluid samples (CSF, Pleural, Peritoneal). These fluids are added to fluorochrome-labeled antibodies that bind specifically to cell surface antigens on lymphocytes. The CD4/CD8 ratio is calculated by dividing the CD4% by the CD8%.
CPT Code(s):	88184 CD3; 88185 CD4; 88185 CD8

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