ARUP's Laboratory Test Directory

DNA Content/Cell Cycle Analysis, Products of Conception : 0093386

Mnemonic: DNA POC

Methodology: Flow Cytometry
Performed: Sun, Tue
Reported: 3-9 days
Specimen Required: Collect:  Products of conception tissue.

Transport:  Paraffin-embedded tissue block with products of conception at 20-25°C.

Remarks:  Include H & E stained slide and surgical pathology report.  If no normal tissue is included in the block, please supply a control tissue block to be processed in parallel.

Unacceptable Conditions:  No tissue remaining on the block.  Samples fixed in Bouin's Solution (picric acid), mercuric chloride containing fixatives (e.g. B5, Zenker's solution) or ethanol-based fixatives containing ethylene glycol, acetic acid, and zinc chloride.  Samples that have been decalcified.

Stability:  Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Interpretive Data: Flow Cytometry can be used to distinguish between partial and complete hydatidiform moles.  Partial moles are usually triploid while complete moles are diploid or tetraploid. (Clinical Medicine: Pathology; 2008: 1; 61-7). Of the 35 cases of histologically apparent partial moles, no complication occurred in those that were triploid.  However, 20% of those that were diploid had complications (persistence, metastasis). [Am J Obstet Gynecol; 1987:157; 969-73].

Please refer to Statement B in the Compliance Statements section in the front of the Laboratory Test Directory.
Note: A thin section of each tissue submitted is stained with H & E.  The DNA content is classified as diploid, triploid, tetraploid or aneuploid.  The DNA index is the ratio of the DNA content of abnormal cells compared to normal cells.
CPT Code(s): 88182
 
 

 

 

 
All ARUP Sites:        www.aruplab.com     ·     www.arupconsult.com     ·     www.arup.utah.edu     ·     www.childx.org     ·     www.utahblood.org