ARUP's Laboratory Test Directory

Estrogens, Fractionated by Tandem Mass Spectrometry : 0093248

Mnemonic: EST FR TMS

Ordering Recommendation: Recommended test for evaluating endogenous estrogen status in postmenopausal women, men, or children.
Methodology: Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry
Performed: Sun-Sat
Reported: 1-4 days
Specimen Required: Collect: Serum separator tube, lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin).

Specimen Preparation: Separate serum or plasma from cells within 2 hours of collection. Transfer 0.5 mL serum or plasma to an ARUP Standard Transport Tube. (Min 0.3 mL)

Storage/Transport Temperature: Refrigerated.

Stability (collection to initiation of testing): After separation from cells: Ambient: 48 hours; Refrigerated: 1 week; Frozen: 1 month
Reference Interval:
Test Number Components Reference Interval
0093247Estradiol, Males, Children or Postmenopausal Females by Tandem Mass Spectrometry
Reference Intervals for Estradiol-Children
Tanner Stages
I
II
III
IV and V 		Males
less than 8 pg/mL
less than 10 pg/mL
1-35 pg/mL
3-35 pg/mL 		Females
less than 56 pg/mL
2-133 pg/mL
12-277 pg/mL
2-259 pg/mL
Age Group
7-9
10-12
13-15
16-17 		Males
less than 7 pg/mL
less than 11 pg/mL
1-36 pg/mL
3-34 pg/mL 		Females
less than 36 pg/mL
1-87 pg/mL
9-249 pg/mL
2-266 pg/mL

Reference Intervals for Estradiol-Adults
Females 18 Years and Older Males 18 years and Older
Pre-menopausal:
Early Follicular: 30-100 pg/mL
Late Follicular: 100-400 pg/mL
Luteal: 50-150 pg/mL 		10-42 pg/mL
Postmenopausal: 2-21 pg/mL
0093249Estrone, by Tandem Mass Spectrometry
Reference Intervals for Estrone-Children
Tanner Stages:
I
II
III
IV and V 		Males
less than 7 pg/mL
less than 11 pg/mL
1-31 pg/mL
2-30 pg/mL 		Females
less than 27 pg/mL
1-39 pg/mL
8-117 pg/mL
4-109 pg/mL
Age Group
7-9
10-12
13-15
16-17 		Males
less than 7 pg/mL
less than 11 pg/mL
1-30 pg/mL
1-32 pg/mL 		Females
less than 20 pg/mL
1-40 pg/mL
8-105 pg/mL
4-133 pg/mL

Reference Intervals for Estrone-Adults
Females 18 Years and Older Males 18 Years and Older
Premenopausal:
Early Follicular: less than 150 pg/mL
Late Follicular: 100-250 pg/mL
Luteal: less than 200 pg/mL 		9-36 pg/mL
Postmenopausal: 3-32 pg/mL
Estrogens Total Calculation
Reference Intervals of Estrogens
Tanner Stages:
I
II
III
IV and V 		Males
1-11
1-19
3-61
4-62 		Females
1-86
3-169
23-351
8-341
Age Group:
7-9
10-12
13-15
16-17 		Males
less than 10
1-19
3-62
4-64 		Females
1-48
2-116
15-333
6-354

Reference Intervals of Estrogens of Adults
Females 18 Years or Older Males 18 Years or Older
Total Estrogens (estrone+ estradiol) pg/mL Total Estrogens (estrone+ estradiol) pg/mL
Pre-menopausal:
Early Follicular: 30-250
Late Follicular: 200-650
Luteal: 50-350 		19-69
Post-menopausal: 5-52

Interpretive Data:

See Compliance Statement B: www.aruplab.com/CS
CPT Code(s): 82671
Cross References: Estrone (E1) and Estradiols (E2) (Estrogens, Fractionated by Tandem Mass Spectrometry), Total Estrogens (Estrogens, Fractionated by Tandem Mass Spectrometry)