ARUP's Laboratory Test Directory

CBFB-MYH11, inv(16) by RT-PCR : 0092209
[ image for: Time Sensitive]
Time Sensitive
[ image for: Additional Technical Information]
Additional Technical Information


Mnemonic: INV(16)

Methodology: Reverse Transcription Polymerase Chain Reaction
Performed: Varies
Reported: 4-7 days
Specimen Required: Collect: Lavender (EDTA) or bone marrow (EDTA).

Specimen Preparation: Transport 5 mL whole blood or 3 mL bone marrow. (Min: 1 mL whole blood or 1 mL bone marrow) Specimens must be received within 48 hours of collection due to lability of RNA.

Storage/Transport Temperature: Refrigerated.

Unacceptable Conditions: Serum or plasma. Frozen or clotted specimens. Specimens collected in anticoagulants other than EDTA. Severely hemolyzed specimens.

Stability (collection to initiation of testing): Ambient: 1 hour; Refrigerated: 48 hours; Frozen: Unacceptable
Interpretive Data: Refer to report.

See Compliance Statement B: www.aruplab.com/CS
Note: CBFB (core binding factor β-chain)-MYH11 (myosin heavy chain 11 gene) fusion transcripts are detected in approximately 10% of de novo acute myelogenous leukemias (AML).  One half of these cases belong to AML subtype M4 with abnormal eosinophils (AML-M4Eo).  The fusion results from a pericentric inversion on chromosome 16 inv(16)(p13q22) or rarely from the translocation t(16;16)(p13;q22).  Based on the breakpoints in the CBFB and MYH11 genes, greater than 95% of analyzed inv(16) cases belong to three types: A (88%), D (5%), and E (5%).
CPT Code(s): 81401, G0452
Cross References: inv(16) (CBFB-MYH11, inv(16) by RT-PCR)