ARUP's Laboratory Test Directory

BK Virus, Quantitative PCR : 0090067

Mnemonic: BK QNT

Methodology: Quantitative Real-Time Polymerase Chain Reaction
Performed: Sun-Sat
Reported: 1-3 days
Specimen Required: Collect: Lavender (EDTA), pink (K2EDTA) or serum separator tube. OR Urine.

Specimen Preparation: Do not freeze whole blood specimens. Transport 1 mL whole blood, serum, plasma or urine in a sterile container. (Min: 0.5 mL).

Storage/Transport Temperature: Refrigerated.

Remarks: Specimen source required.

Unacceptable Conditions: Frozen whole blood. Heparinized specimens.

Stability (collection to initiation of testing): Ambient: 24 hours; Refrigerated: 72 hours; Frozen: 1 month
Reference Interval:
Not detected
Interpretive Data: The quantitative range of this assay is 2.6-8.6 log copies/mL (390-390,000,000 copies/mL).

A negative result (less than 2.6 log copies/mL or less than 390 copies/mL) does not rule out the presence of PCR inhibitors in the patient specimen or BK virus DNA concentrations below the level of detection of the assay. Inhibition may also lead to underestimation of viral quantitation.

No international standard is currently available for calibration of this assay. Caution should be taken when interpreting results generated by different assay methodologies.

See Compliance Statement A: www.aruplab.com/CS
Note: The limit of quantification for this DNA assay is 2.6 log copies/mL (390 copies/mL). If the assay DID NOT DETECT the virus, the test result will be reported as "< 2.6 log copies/mL (< 390 copies/mL)." If the assay DETECTED the presence of the virus but was not able to accurately quantify the number of copies, the test result will be reported as "Not Quantified."
CPT Code(s): 87799
Cross References: BK (Polyoma) Virus (BK Virus, Quantitative PCR)