ARUP's Laboratory Test Directory

Testosterone Free, Females or Children : 0081059

Mnemonic: TESTOS FR

Ordering Recommendation: Most sensitive test for detection of hyperandrogenemia in women and children. Acceptable test for androgen deficiency in men.
Methodology: Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry/Electrochemiluminescent Immunoassay
Total Testosterone and SHBG are measured and free testosterone is estimated from these measurements.
Performed: Sun-Sat
Reported: 1-4 days
Specimen Required: Patient Prep: Collect between 6-10 a.m.

Collect: Serum separator tube or green (sodium or lithium heparin).

Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.4 mL)

Storage/Transport Temperature: Refrigerated.

Remarks: This test is suggested for women and children due to an improved sensitivity of testosterone by LC-MS/MS.

Unacceptable Conditions: EDTA plasma.

Stability (collection to initiation of testing): After separation from cells: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 6 months
Reference Interval:
Female Free Testosterone, pg/mL Male Free Testosterone, pg/mL
1-6 years: Less than 0.6 pg/mL
7-9 years: 0.6-1.8 pg/mL
10-11 years: 0.1-3.5 pg/mL
12-13 years: 0.9-6.8 pg/mL
14-15 years: 1.2-7.5 pg/mL
16-17 years: 1.2-9.9 pg/mL
18-30 years: 0.8-7.4 pg/mL
31-40 years: 1.3-9.2 pg/mL
41-51 years: 1.1-5.8 pg/mL
Postmenopausal: 0.6-3.8 pg/mL
Tanner Stage I: Less than 2.2 pg/mL
Tanner Stage II: 0.4-4.5 pg/mL
Tanner Stage III: 1.3-7.5 pg mL
Tanner Stage IV: 1.1-15.5 pg/mL
Tanner Stage V: 0.8-9.2 pg/mL 		1-6 years: Less than 0.6 pg/mL
7-9 years: 0.1-0.9 pg/mL
10-11 years: 0.1-6.3 pg/mL
12-13 years: 0.5-98.0 pg/mL
14-15 years: 3-138.0 pg/mL
16-17 years: 38.0-173.0 pg/mL
18 years and older: 47-244 pg/mL
Tanner Stage I: Less than or equal to 3.7 pg/mL
Tanner Stage II: 0.3-21 pg/mL
Tanner Stage III: 1.0-98.0 pg mL
Tanner Stage IV: 35.0-169.0 pg/mL
Tanner Stage V: 41.0-239.0 pg/mL

Interpretive Data: The concentration of free testosterone is derived from a mathematical expression based on the constant for the binding of testosterone to SHBG.

See Compliance Statement B: www.aruplab.com/CS
Note: This test is suggested for women and children due to the improved sensitivity of testosterone by LC-MS/MS. Testosterone Free, Females and Children by tandem mass spectrometry, only provides a result for the free testosterone value (pg/mL).
CPT Code(s): 84402