ARUP's Laboratory Test Directory

Testosterone, Bioavailable & Sex Hormone Binding Globulin (Includes Total Testosterone), Females or Children : 0081057

Mnemonic: BIO T MASS

Methodology: High Performance Liquid Chromatography/Tandem Mass Spectrometry/Electrochemiluminescent Immunoassay
The concentrations of free and bioavailable testosterone are derived from mathematical expressions based on constants for the binding of testosterone to albumin and/or sex hormone binding globulin.
Performed: Sun-Sat
Reported: 1-4 days
Specimen Required: Collect:  One 6 mL serum separator tube or green (sodium or lithium heparin). Specimen should be collected between 6-10 a.m.

Transport:  1 mL serum or plasma at 2-8°C. (Min: 0.4 mL) Also acceptable: plasma (EDTA or heparin).  Submit specimen in an ARUP Standard Transport Tube.

Remarks:  Separate serum from cells ASAP.  This test is suggested for women and children due to an improved sensitivity of testosterone by LC-MS/MS.

Stability:  After separation from cells: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 6 months
Reference Interval:
 
Test Number Components Reference Interval
0099375Sex Hormone Binding Globulin
 
Age Male Female
1-30 days
31-364 days
1-3 years
4-6 years
7-9 years
10-12 years
13-15 years
16-17 years
18 years and older 		13-85 nmol/L
70-250 nmol/L
50-180 nmol/L
45-175 nmol/L
28-190 nmol/L
23-160 nmol/L
13-140 nmol/L
10-60 nmol/L
11-80 nmol/L 		14-60 nmol/L
60-215 nmol/L
60-190 nmol/L
55-170 nmol/L
35-170 nmol/L
17-155 nmol/L
11-120 nmol/L
19-145 nmol/L
30-135 nmol/L
Tanner Stage I
Tanner Stage II
Tanner Stage III
Tanner Stage IV
Tanner Stage V 		26-286 nmol/L
22-169 nmol/L
13-104 nmol/L
11-60 nmol/L
11-71 nmol/L 		30-173 nmol/L
16-127 nmol/L
12-98 nmol/L
14-151 nmol/L
23-165 nmol/L
 Testosterone LC-MS, Bioavailable  
 
Female - Bioavailable Testosterone, ng/dL Male - Bioavailable Testosterone, ng/dL
1-6 years: Less than 1.3 ng/dL
7-9 years: 0.3-5.0 ng/dL
10-11 years: 0.4-9.6 ng/dL
12-13 years: 1.7-18.8 ng/dL
14-15 years: 3.0-22.6 ng/dL
16-17 years: 3.3-28.6 ng/dL
18-30 years: 2.2-20.6 ng/dL
31-40 years: 4.1-25.5 ng/dL
41-51 years: 2.8-16.5 ng/dL
Postmenopausal: 1.5-9.4 ng/dL
Tanner Stage I: 0.3-5.5 ng/dL
Tanner Stage II: 1.2-15.0 ng/dL
Tanner Stage III: 3.8-28.0 ng/dL
Tanner Stage IV: 2.8-39.0 ng/dL
Tanner Stage V: 2.5-23.0 ng/dL 		1-6 years: Less than 1.3 ng/dL
7-9 years: 0.3-2.8 ng/dL
10-11 years: 0.1-17.9 ng/dL
12-13 years: 1.4-288.0 ng/dL
14-15 years: 9.5-337.0 ng/dL
16-17 years: 35.0-509.0 ng/dL
18 years and older: 130-680 ng/dL
Tanner Stage I: 0.3-13.0 ng/dL
Tanner Stage II: 0.3-59.0 ng/dL
Tanner Stage III: 1.9-296.0 ng/dL
Tanner Stage IV: 40.0-485.0 ng/dL
Tanner Stage V: 124.0-596.0 ng/dL
0081059Testosterone Free, Females or Children  
 
Female Free Testosterone, pg/mL Male Free Testosterone, pg/mL
1-6 years: Less than 0.6 pg/mL
7-9 years: 0.6-1.8 pg/mL
10-11 years: 0.1-3.5 pg/mL
12-13 years: 0.9-6.8 pg/mL
14-15 years: 1.2-7.5 pg/mL
16-17 years: 1.2-9.9 pg/mL
18-30 years: 0.8-7.4 pg/mL
31-40 years: 1.3-9.2 pg/mL
41-51 years: 1.1-5.8 pg/mL
Postmenopausal: 0.6-3.8 pg/mL
Tanner Stage I: Less than 2.2 pg/mL
Tanner Stage II: 0.4-4.5 pg/mL
Tanner Stage III: 1.3-7.5 pg mL
Tanner Stage IV: 1.1-15.5 pg/mL
Tanner Stage V: 0.8-9.2 pg/mL 		1-6 years: Less than 0.6 pg/mL
7-9 years: 0.1-0.9 pg/mL
10-11 years: 0.1-6.3 pg/mL
12-13 years: 0.5-98.0 pg/mL
14-15 years: 3-138.0 pg/mL
16-17 years: 38.0-173.0 pg/mL
18 years and older: 47-244 pg/mL
Tanner Stage I: Less than or equal to 3.7 pg/mL
Tanner Stage II: 0.3-21 pg/mL
Tanner Stage III: 1.0-98.0 pg mL
Tanner Stage IV: 35.0-169.0 pg/mL
Tanner Stage V: 41.0-239.0 pg/mL
0081058Testosterone, Females or Children  
 
Serum Total Testosterone, Determined by LC-MS/MS
Female Male
Premature (26-28 weeks): 5-16 ng/dL
Premature (31-35 weeks): 5-22 ng/dL
Newborn: 20-64 ng/dL
1-7 months: Levels decrease during the first month to less than 10 ng/dL and remain at this level until puberty.
7-9 years: Less than 15 ng/dL
10-11 years: 2-42 ng/dL
12-13 years: 6-64 ng/dL
14-15 years: 9-49 ng/dL
16-17 years: 8-63 ng/dL
18-30 years: 11-59 ng/dL
31-40 years: 11-56 ng/dL
41-51 years: 9-55 ng/dL
Postmenopausal: 6-25 ng/dL
Tanner Stage I: Less than 17 ng/dL
Tanner Stage II: 4-39 ng/dL
Tanner Stage III: 10-60 ng/dL
Tanner Stage IV: 8-63 ng/dL
Tanner Stage V: 10-60 ng/dL 		Premature (26-28 weeks): 59-125 ng/dL
Premature (31-35 weeks): 37-198 ng/dL
Newborn: 75-400 ng/dL
1-7 months: Levels decrease rapidly the first week to 20-50 ng/dL, and then increase to 60-400 ng/dL between 20-60 days.  Levels then decline to pre-pubertal range levels of 3-10 ng/dL by seven months.
7-9 years: Less than 9 ng/dL
10-11 years: 2-57 ng/dL
12-13 years: 7-747 ng/dL
14-15 years: 33-585 ng/dL
16-17 years: 185-886 ng/dL
18-39 years: 400-1080 ng/dL
40-59 years: 350-890 ng/dL
60 years and older: 350-720 ng/dL
Tanner Stage I: Less than 20 ng/dL
Tanner Stage II: 2-149 ng/dL
Tanner Stage III: 7-762 ng/dL
Tanner Stage IV: 164-854 ng/dL
Tanner Stage V: 194-783 ng/dL

Interpretive Data: The concentrations of free and bioavailable testosterone are derived from mathematical expressions based on constants for the binding of testosterone to albumin and/or sex hormone binding globulin.
Note: This test is suggested for women and children due to an improved sensitivity of testosterone by LC-MS/MS.
CPT Code(s): 84403 Total testosterone; 84270 SHBG
 
 

 

 

 
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