ARUP's Laboratory Test Directory

Testosterone, Free and Total (Includes Sex Hormone Binding Globulin), Females or Children : 0081056

Mnemonic: TESTOS F&T

Ordering Recommendation: Recommended initial test in the evaluation of suspected hyperandrogenemia in women and children. Acceptable test for evaluating androgen deficiency in men.
Methodology: Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry/Electrochemiluminescent Immunoassay
The concentration of free testosterone is derived from a mathematical expression based on the constant for the binding of testosterone to sex hormone binding globulin.
Performed: Sun-Sat
Reported: 1-4 days
Specimen Required: Patient Prep: Collect between 6-10 a.m.

Collect: Serum separator tube or green (sodium or lithium heparin).

Specimen Preparation: Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.6 mL)

Storage/Transport Temperature: Refrigerated.

Remarks: This test is suggested for women and children due to an improved sensitivity of testosterone by LC-MS/MS.

Unacceptable Conditions: EDTA plasma.

Stability (collection to initiation of testing): After separation from cells: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 6 months
Reference Interval:
Test Number Components Reference Interval
0081059Testosterone Free, Females or Children
Female Free Testosterone, pg/mL Male Free Testosterone, pg/mL
1-6 years: Less than 0.6 pg/mL
7-9 years: 0.6-1.8 pg/mL
10-11 years: 0.1-3.5 pg/mL
12-13 years: 0.9-6.8 pg/mL
14-15 years: 1.2-7.5 pg/mL
16-17 years: 1.2-9.9 pg/mL
18-30 years: 0.8-7.4 pg/mL
31-40 years: 1.3-9.2 pg/mL
41-51 years: 1.1-5.8 pg/mL
Postmenopausal: 0.6-3.8 pg/mL
Tanner Stage I: Less than 2.2 pg/mL
Tanner Stage II: 0.4-4.5 pg/mL
Tanner Stage III: 1.3-7.5 pg mL
Tanner Stage IV: 1.1-15.5 pg/mL
Tanner Stage V: 0.8-9.2 pg/mL 		1-6 years: Less than 0.6 pg/mL
7-9 years: 0.1-0.9 pg/mL
10-11 years: 0.1-6.3 pg/mL
12-13 years: 0.5-98.0 pg/mL
14-15 years: 3-138.0 pg/mL
16-17 years: 38.0-173.0 pg/mL
18 years and older: 47-244 pg/mL
Tanner Stage I: Less than or equal to 3.7 pg/mL
Tanner Stage II: 0.3-21 pg/mL
Tanner Stage III: 1.0-98.0 pg mL
Tanner Stage IV: 35.0-169.0 pg/mL
Tanner Stage V: 41.0-239.0 pg/mL
0081058Testosterone, Females or Children Effective November 14, 2011
Serum Total Testosterone, Determined by LC-MS/MS
Female Male
Premature (26-28 weeks): 5-16 ng/dL
Premature (31-35 weeks): 5-22 ng/dL
Newborn: 20-64 ng/dL
1-5 months: Less than 20 ng/dL
6-24 months: Less than 9 ng/dL
2-3 years: Less than 20 ng/dL
4-5 years: Less than 30 ng/dL
6-7 years: Less than 7 ng/dL
8-9 years: 1-11ng/dL
10-11 years: 3-32 ng/dL
12-13 years: 6-50 ng/dL
14-15 years: 6-52 ng/dL
16-17 years: 9-58 ng/dL
18-39 years: 9-55 ng/dL
40-59 years: 9-55 ng/dL
60 years and older: 5-32 ng/dL
Premenopausal (Greater than 18 years): 9-55 ng/dL
Postmenopausal: 5-32 ng/dL
Tanner Stage I: 2-17 ng/dL
Tanner Stage II: 5-40 ng/dL
Tanner Stage III: 10-63 ng/dL
Tanner Stage IV-V: 11-62 ng/dL 		Premature (26-28 weeks): 59-125 ng/dL
Premature (31-35 weeks): 37-198 ng/dL
Newborn: 75-400 ng/dL
1-5 months: 14-363 ng/dL
6-24 months: Less than 37 ng/dL
2-3 years: Less than 15 ng/dL
4-5 years: Less than 19 ng/dL
6-7 years: Less than 13 ng/dL
8-9 years: 2-8 ng/dL
10-11 years: 2-165 ng/dL
12-13 years: 3-619 ng/dL
14-15 years: 31-733 ng/dL
16-17 years: 158-826 ng/dL
18-39 years: 300-1080 ng/dL
40-59 years: 300-890 ng/dL
60 years and older: 300-720 ng/dL
Tanner Stage I: 2-15 ng/dL
Tanner Stage II: 3-303 ng/dL
Tanner Stage III: 10-851 ng/dL
Tanner Stage IV-V: 162-847 ng/dL
0099375Sex Hormone Binding Globulin
Age Male Female
1-30 days
31-364 days
1-3 years
4-6 years
7-9 years
10-12 years
13-15 years
16-17 years
18 years and older 		13-85 nmol/L
70-250 nmol/L
50-180 nmol/L
45-175 nmol/L
28-190 nmol/L
23-160 nmol/L
13-140 nmol/L
10-60 nmol/L
11-80 nmol/L 		14-60 nmol/L
60-215 nmol/L
60-190 nmol/L
55-170 nmol/L
35-170 nmol/L
17-155 nmol/L
11-120 nmol/L
19-145 nmol/L
30-135 nmol/L
Tanner Stage I
Tanner Stage II
Tanner Stage III
Tanner Stage IV
Tanner Stage V 		26-186 nmol/L
22-169 nmol/L
13-104 nmol/L
11-60 nmol/L
11-71 nmol/L 		30-173 nmol/L
16-127 nmol/L
12-98 nmol/L
14-151 nmol/L
23-165 nmol/L

Interpretive Data: The concentration of free testosterone is derived from a mathematical expression based on the constant for the binding of testosterone to sex hormone binding globulin.

See Compliance Statement B: www.aruplab.com/CS
Note: This test is suggested for women and children due to an improved sensitivity of testosterone by LC-MS/MS.  Please refer to individual components for stability of sample for this test.
CPT Code(s): 84403; 84270
Cross References: Testosterone Free (Testosterone, Free & Total (Includes Sex Hormone Binding Globulin), Females or Children)