ARUP's Laboratory Test Directory

Vitamin B1 (Thiamine), Whole Blood : 0080388

Mnemonic: VIT B1 WB

Ordering Recommendation: Do not use to monitor thiamine supplementation. Use to determine thiamine deficiency.
Methodology: Quantitative High Performance Liquid Chromatography
Performed: Sun-Sat
Reported: 2-6 days
Specimen Required: Collect: Green (sodium or lithium heparin), lavender (EDTA), or pink (K2EDTA).

Specimen Preparation: Protect from light within one hour of collection and during storage and shipment. Transfer 3 mL whole blood to an ARUP Amber Transport Tube. (Min: 0.6 mL)

Storage/Transport Temperature: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.

Unacceptable Conditions: Any specimen other than whole blood. Glass tubes. Clotted or non-frozen specimens.

Stability (collection to initiation of testing): Ambient: Unacceptable; Refrigerated: 4 hours; Frozen: 6 months
Reference Interval:
70-180 nmol/L
Interpretive Data: The concentration of thiamine diphosphate (TDP), the primary active form of vitamin B1, is measured in this assay. Approximately 90% of vitamin B1 present in whole blood is TDP. Thiamine and thiamine monophosphate, which comprise the remaining 10%, are not measured.

See Compliance Statement B: www.aruplab.com/CS
Note: Whole blood is the preferred specimen for thiamine assessment.  Approximately 80% of thiamine present in whole blood is found in red blood cells. 
CPT Code(s): 84425
Cross References: B1 Vitamin (Vitamin B1 (Thiamine), Whole Blood) , TDP (Vitamin B1 (Thiamine), Whole Blood) , Thiamine (Vitamin B1 (Thiamine), Whole Blood) , Thiamine Diphosphate (Vitamin B1 (Thiamine), Whole Blood)