Ordering Recommendation

Use for nutritional assessment of vitamin B1 (thiamine).

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation
Collect

Green (sodium or lithium heparin), lavender (EDTA), or pink (K2EDTA).

Specimen Preparation

Transfer 3 mL whole blood to an ARUP standard transport tube (Min: 0.6 mL) and freeze within 1 hour of collection.

Storage/Transport Temperature

Frozen

Unacceptable Conditions

Any specimen other than whole blood. Plasma separator tubes. Glass tubes. Clotted or nonfrozen specimens.

Remarks
Stability

Room Temperature: Unacceptable; Refrigerated: Unacceptable; Frozen: 6 months

Methodology

Quantitative High Performance Liquid Chromatography (HPLC)/Liquid Chromatography-Tandem Mass Spectrometry

Performed

Sun-Sat

Reported

2-5 days

Reference Interval

70-180 nmol/L

Interpretive Data

This assay measures the concentration of thiamine diphosphate (TDP), the primary active form of vitamin B1. Approximately 90 percent of vitamin B1 present in whole blood is TDP. Thiamine and thiamine monophosphate, which comprise the remaining 10 percent, are not measured.

Compliance Category

Laboratory Developed Test (LDT)

Note

Whole blood is the preferred specimen for thiamine assessment. Approximately 80 percent of thiamine present in whole blood is found in red blood cells. 

Hotline History

N/A

CPT Codes

84425

Components

Component Test Code* Component Chart Name LOINC
0080388 Vitamin B1, Whole Blood 74444-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • B1 Vitamin
  • TDP
  • Thiamine
  • Thiamine Diphosphate
  • Thiamine Pyrophosphate
  • TPP
  • VITB1
Vitamin B1 (Thiamine), Whole Blood