ARUP's Laboratory Test Directory

Vitamin D, 25-Hydroxy : 0080379
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Additional Technical Information
  


Mnemonic: VIT D 25

Ordering Recommendation: Use to diagnose vitamin D insufficiency and monitor response to vitamin D therapy. Testing is recommended only for patients at risk for vitamin D insufficiency.
Methodology: Quantitative Chemiluminescent Immunoassay
Performed: Sun-Sat
Reported: Within 24 hours
Specimen Required: Collect: Serum separator tube. Also acceptable: Green (sodium or lithium heparin).

Specimen Preparation: Transfer 1 mL serum or heparinized plasma to an ARUP Standard Transport Tube. (Min: 0.3 mL)

Storage/Transport Temperature: Refrigerated.

Unacceptable Conditions: Tissue or urine. Grossly hemolyzed or lipemic specimens.

Stability (collection to initiation of testing): After separation from cells: Ambient: 72 hours; Refrigerated: 1 week; Frozen: 6 months
Reference Interval:
 
0-17 years
Deficiency Less than 20 ng/mL
Optimum level Greater than or equal to 20 ng/mL*
*(Wagner CL et al. Pediatrics 2008; 122: 1142-52.)

 
18 years and older
Deficiency Less than 20 ng/mL
Insufficiency 20-29 ng/mL
Optimum Level 30-80 ng/mL
Possible Toxicity Greater than 150 ng/mL

Interpretive Data: This assay accurately quantifies the sum of vitamin D3, 25-Hydroxy and vitamin D2, 25-Hydroxy. 
CPT Code(s): 82306
Cross References: D, 25-Hydroxy, Vitamin (Vitamin D, 25-Hydroxy)