| Methodology: |
Quantitative Enzymatic
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| Performed: |
Sun-Sat
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| Reported: |
1-2 days
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| Specimen Required: |
Collect: Whole blood using either a syringe or green (sodium or lithium heparin), lavender (EDTA), or pink (K2EDTA).
Specimen Preparation: 1) Immediately after blood is drawn, add exactly 1 mL whole blood to a chilled pyruvate collection tube containing 2 mL 8 percent (w/v) perchloric acid. (ARUP supply #16567). Available online through eSupply using ARUP Connect™ or contact Client Services at (800) 522-2787.
2) Mix well for 30 seconds then place in an ice bath for 10 minutes.
3) Centrifuge for 10 minutes at 1500 x g.
4) Decant 2 mL supernatant to an ARUP Standard Transport Tube and freeze. (Min: 1 mL)
Storage/Transport Temperature: Frozen.
Unacceptable Conditions: If less than 1 mL of blood is added to collection tube, pH of the supernatant will be too low for testing to be performed.
Stability (collection to initiation of testing): Ambient: 8 hours; Refrigerated: 1 month; Frozen: 6 months
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