ARUP's Laboratory Test Directory

Vitamin D, 25-Hydroxy by RIA : 0070277

Mnemonic: D25

Methodology: Radioimmunoassay
Performed: Tue, Fri
Reported: 2-5 days
Specimen Required: Patient Prep:  Fasting recommended but not required.

Collect:  One 4 mL serum separator tube.   Also acceptable: lavender (EDTA), pink (K2 EDTA), or green (sodium or lithium heparin).

Storage/Transport Temperature:  1 mL serum, frozen. (Min: 0.2 mL)  Submit specimen in an ARUP Standard Transport Tube.

Stability (collection to initiation of testing):  After separation from cells: Ambient: 3 days; Refrigerated: 1 week; Frozen: 3 months
Reference Interval:
0-17 years:Deficiency: less than 20 ng/mL
Optimum level: greater than or equal to 20 ng/mL*
*(Wagner CL et al. Pediatrics 2008; 122: 1128-38.)

18 years and older:
Deficiency:  Less than 20 ng/mL
Insufficiency:  20-29 ng/mL
Optimum Level:  30-80 ng/mL
Possible Toxicity:  Greater than 150 ng/mL
Interpretive Data: This assay accurately quantifies the sum of vitamin D3, 25-Hydroxy and vitamin D2, 25-Hydroxy. 
CPT Code(s): 82306
 
 

 

 

 
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