ARUP's Laboratory Test Directory

Cyclosporine A by Tandem Mass Spectrometry : 0070035

Mnemonic: CYA

Methodology: Quantitative Liquid Chromatography-Tandem Mass Spectrometry
Performed: Sun-Sat
Reported: Within 24 hours
Specimen Required: Patient Prep: Pre-dose (trough) levels should be drawn.

Collect: Lavender (EDTA) or pink (K2EDTA).

Specimen Preparation: Transfer 1 mL well-mixed whole blood to a plastic vial. (Min: 0.25 mL)

Storage/Transport Temperature: Refrigerated.

Unacceptable Conditions: Serum or plasma. Specimens left at room temperature for longer than 24 hours. Clotted specimens.

Stability (collection to initiation of testing): Ambient: 24 hours; Refrigerated: 2 weeks; Frozen: 2 months
Reference Interval:
Therapeutic Range:
Kidney and heart transplant patients:
Up to 3 months post-transplant: 350-525 ng/mL
4 months and older post-transplant: 145-350 ng/mL
 
Liver transplant patients:
290-525 ng/mL
Toxic alert value: greater than 700 ng/mL
Note: Cyclosporine (Sandimmune®) whole blood concentrations can be measured by either chromatographic or immunoassay methodologies.  These two methodologies are not directly interchangeable, and the measured cyclosporine whole blood concentration depends upon the type of assay used.  Reference ranges may vary according to the specific immunoassay or LC/MS/MS test used. Generally, immunoassays have been reported to have a positive bias relative to the reference assay, HPLC with detection by tandem mass spectrometry (LC/MS/MS).
CPT Code(s): 80158
Cross References: Cyclosporine (Cyclosporine A by Tandem Mass Spectrometry), Gengraf® (Cyclosprorine A by Tandem Mass Spectrometry) , Neoral® (Cyclosporine A by Tandem Mass Spectrometry) , Restasis (Cyclosporine A by Tandem Mass Spectrometry), Sandimmune® (Cyclosporine A by Tandem Mass Spectrometry)