ARUP's Laboratory Test Directory

Viral Culture & Cytomegalovirus Rapid Culture : 0065034

Mnemonic: V VIC

Methodology: Cell Culture/Immunofluorescence
Performed: Sun-Sat
Reported: Viral Cutlure: 3-16 days
Cytomegalovirus Rapid Culture: 1-5 days
Specimen Required: Collect: Body fluids, eye swab, lesion, respiratory specimen (bronchoalveolar lavage (BAL), nasopharyngeal aspirate, swab, or washing, or tracheal aspirate) tissue biopsy, or urine.

Specimen Preparation: Do not freeze.
Nasopharyngeal Washing or Aspirate, Urine, or Tracheal Aspirate:
Immediately transfer to sterile, leak-proof container.
Swabs or Tissue:
Immediately transfer to viral transport media.
Place each specimen in a separate, individually sealed bag.


Storage/Transport Temperature: Refrigerated.

Submit specimen according to Biological Substance, Category B, shipping guidelines.


Remarks: Specimen source required. Antigen detection and culture are available for specific viruses.

Unacceptable Conditions: Calcium alginate, dry, or wood swabs.

Stability (collection to initiation of testing): Ambient: 2 hours; Refrigerated: 72 hours; Frozen: Unacceptable
Reference Interval:
Negative - No virus isolated.
Note: The following tests are standard of care for diagnosing viral infection in CSF specimens:
Cytomegalovirus by PCR (0060040)
Enterovirus Detection by RT-PCR (0050249)
Herpes Simplex Virus by PCR (0060041)
Varicella-Zoster Virus by PCR (0060042).


The viruses that can be isolated include: enteroviruses, herpes simplex virus, influenza A & B, parainfluenza types 1, 2, 3, adenovirus, varicella-zoster virus, cytomegalovirus, and RSV.  However, virus specific tests are recommended for varicella-zoster virus, order Varicella-Zoster Virus DFA with Reflex to Varicella-Zoster Virus Culture (0060282); and RSV order Respiratory Syncytial Virus DFA Stain (0060288). Antigen detection is available for respiratory viruses, order Respiratory Viruses DFA Stain (0060289); and may be requested in conjunction with culture.

An ELISA test (Rotavirus & Adenovirus 40-41 Antigens (0065067)) is available for enteric adenoviruses 40 & 41 and rotavirus in stool specimens.  For measles virus culture, order Measles (Rubeola) Virus Culture (0065055).  For mumps virus culture, order Mumps Virus Culture (0065056). 

If a specific virus is suspected, please indicate which virus on the test request.
CPT Code(s): 87252 Tissue Culture; 87254 Shell vial
Cross References: Culture, Viral with CMV (Viral Culture & Cytomegalovirus Rapid Culture)
 
 

 

 

 
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