ARUP's Laboratory Test Directory

0065034: Viral Culture & Cytomegalovirus Rapid Culture

Test Mnemonic: V VIC
Methodology: Cell Culture/Immunofluorescence

Performed: Sun-Sat

Reported: Viral Cutlure: 3-16 days
Cytomegalovirus Rapid Culture: 1-5 days

Specimen Required:  
Collect: Eye swab, nasopharyngeal aspirate/swab/washing, throat swab, tissue, biopsy, lesion, tracheal aspirate, or urine. Source of specimen is required.

Transport: Nasopharyngeal aspirate/washing, urine, or tracheal aspirate in a sterile, leak-proof container at 2-8°C. Eye swab, nasopharyngeal swab, throat swab, or tissue in viral transport media at 2-8°C.  Place each specimen in a separate, individually sealed bag.

Submit specimen according to Biological Substance, Category B, shipping guidelines.

Pediatric Collection/Transport:

Submit specimen according to Biological Substance, Category B, shipping guidelines.

Remarks: Do not freeze.  Transfer specimen to transport media as soon as possible after collection. Antigen detection and culture are available for specific viruses.

Unacceptable Conditions: Stool, rectal swab and CSF samples.   Dry swabs, wood swabs, and calcium alginate swabs. 

Stability: Ambient: 2 hours; Refrigerated: 3 days; Frozen: Unacceptable

Reference Interval:
Negative - No virus isolated.

Note:
The following tests are standard of care for diagnosing viral infection in CSF specimens:
Cytomegalovirus by PCR (0060040)
Enterovirus Detection by RT-PCR (0050249)
Herpes Simplex Virus by PCR (0060041)
Varicella-Zoster Virus by PCR (0060042).


The viruses that can be isolated include: enteroviruses, herpes simplex virus, influenza A & B, parainfluenza types 1, 2, 3, adenovirus, varicella-zoster virus, cytomegalovirus, and RSV.  However, virus specific tests are recommended for varicella-zoster virus, order Varicella-Zoster Virus DFA with Reflex to Varicella-Zoster Virus Culture (0060282); and RSV order Respiratory Syncytial Virus DFA Stain (0060288). Antigen detection is available for respiratory viruses, order Respiratory Viruses DFA Stain (0060289); and may be requested in conjunction with culture.

An ELISA test (Rotavirus & Adenovirus 40-41 Antigens (0065067)) is available for enteric adenoviruses 40 & 41 and rotavirus in stool specimens.  For measles virus culture, order Measles (Rubeola) Virus Culture (0065055).  For mumps virus culture, order Mumps Virus Culture (0065056). 

If a specific virus is suspected, please indicate which virus on the test request.

CPT Code(s):
87252 Tissue Culture; 87254 Shell vial

 

 

 
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