ARUP's Laboratory Test Directory

Acid-Fast Bacillus (AFB) Identification : 0060999
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Additional Technical Information
  


Mnemonic: MC AFBID

Methodology: Identification. Methods may include biochemical, nucleic acid probe, polymerase chain reaction, or sequencing.
Performed: Sun-Sat
Reported: Varies (depends on organism growth)
Specimen Required: Collect: Actively growing organism, in pure culture.

Specimen Preparation: Transport sealed container with pure culture on solid or in liquid media. Place each specimen in an individually sealed bag.

Storage/Transport Temperature: Room temperature. Submit specimen according to Infectious Substance, Category A, shipping guidelines.

Submit specimen according to Infectious Substance, Category A, shipping guidelines.

Remarks: Indicate source of organism and any other pertinent information, such as the growth rate of the organism or DNA probe results. Probes can be performed directly from Middlebrook 7H9 broth. MGIT is not suitable for direct probe testing.

Unacceptable Conditions: Mixed cultures or non-viable organisms. Organisms submitted on an agar plate.

Stability (collection to initiation of testing): Ambient: 2 weeks; Refrigerated: 2 weeks; Frozen: 2 weeks
Reference Interval:
Complete identification of clinically significant isolates.
Interpretive Data: For identification by DNA sequencing:

See Compliance Statement B: www.aruplab.com/CS
Note: A handling fee will be billed for all organisms submitted that are not in pure culture as requested in the specimen requirements.
Identification of AFB isolates is billed by individual probes and tests performed.
CPT Code(s): CPT codes for identification vary based on method.
Cross References: Acid-fast Organism Identification by DNA Probe and/or Sequencing (AFB Identification), Culture Referred for Identification, Mycobacterium (AFB Identification), Identification, Mycobacteria (AFB Identification), Organism Identification (AFB Identification)