ARUP's Laboratory Test Directory
| 0060999: AFB Identification |
| Test Mnemonic: MC AFBID | |
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Methodology: Standard reference procedures for identification of AFB. DNA probes available for M. tuberculosis complex and M. avium-intracellulare complex. DNA sequencing and other molecular techniques are used for other mycobacteria species.
Performed: Sun-Sat Reported: Varies (depends on organism growth) |
| Specimen Required: | |
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Collect: Viable organism in pure culture on solid or in liquid media.
Transport: Viable organism in sealed container at 20-25°C. Submit specimen according to Infectious Substance, Category A, shipping guidelines. Pediatric Collection/Transport: Submit specimen according to Infectious Substance, Category A, shipping guidelines. Remarks: Indicate source of organism and any other pertinent information. Additional information to include when submitting an isolate for identification: growth rate of the organism, DNA probe results. Probes can be performed directly from Middlebrook 7H9 broth. MGIT is not suitable for direct probe testing. Unacceptable Conditions: Nonviable organisms, mixed cultures, leaking containers, or organisms submitted on an agar plate. Stability: Ambient: 2 weeks; Refrigerated: 2 weeks; Frozen: 2 weeks |
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| Reference Interval: |
| Complete identification of clinically significant isolates. |
| Interpretive Data: | |
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For identification by DNA sequencing:
Please refer to Statement B in the Compliance Statements section in the front of the Laboratory Test Directory. |
| Note: | |
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A handling fee will be billed for all organisms submitted that are not in pure culture as requested in the specimen requirements. Identification of AFB isolates is billed by individual probes and tests performed. |
| CPT Code(s): | |
| CPT codes for identification vary based on method. |