| Methodology: |
Standard reference procedures for identification of AFB. DNA probes available for M. tuberculosis complex and M. avium-intracellulare complex. DNA sequencing and other molecular techniques are used for other mycobacteria species.
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| Performed: |
Sun-Sat
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| Reported: |
Varies (depends on organism growth)
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| Specimen Required: |
Collect: Viable organism in pure culture on solid or in liquid media.
Transport: Viable organism in sealed container at 20-25°C.
Submit specimen according to Infectious Substance, Category A, shipping guidelines.
Pediatric Collection/Transport:
Submit specimen according to Infectious Substance, Category A, shipping guidelines.
Remarks: Indicate source of organism and any other pertinent information. Additional information to include when submitting an isolate for identification: growth rate of the organism, DNA probe results.
Probes can be performed directly from Middlebrook 7H9 broth. MGIT is not suitable for direct probe testing.
Unacceptable Conditions: Nonviable organisms, mixed cultures, leaking containers, or organisms submitted on an agar plate.
Stability: Ambient: 2 weeks; Refrigerated: 2 weeks; Frozen: 2 weeks
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