ARUP's Laboratory Test Directory

Chlamydia trachomatis DFA : 0060287

Mnemonic: CHLAF

Ordering Recommendation: Not recommended for detecting Chlamydia trachomatis. Preferred test is one of the nucleic acid amplification tests (NAAT) [ie, Chlamydia trachomatis by Transcription-Mediated Amplification (TMA) (0060243) or Chlamydia trachomatis and Neisseria gonorrhoeae by by Transcription-Mediated Amplification (TMA) (0060241)].
Methodology: Direct Fluorescent Antibody Stain
Performed: Sun-Sat
Reported: Within 24 hours
Specimen Required: Collect: Eye, genital, nasopharyngeal, or rectal swab.
For Chlamydia DFA only, collection kits with fixative are available (ARUP supply #16578). Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.

Specimen Preparation: Fix slide as directed by collection kit. Transport fixed slide.

Storage/Transport Temperature: Room temperature.

Remarks: Specimen source preferred.

Unacceptable Conditions: Swabs in gel. Transport systems designed for use with other methodologies of Chlamydia testing. Dry swabs.

Stability (collection to initiation of testing): Fixed Slide: Ambient: 1 week; Refrigerated: 1 week; Frozen: 1 week
Reference Interval:
Negative
Note: Sensitivity of this method is dependent on adequacy of the smear. If there are fewer than 20 columnar epithelial cells, the result will be reported as "sample inadequate."

Amplified DNA testing is recommended for detection of Chlamydia trachomatis from endocervical or urethral specimens.  Refer to Chlamydia trachomatis by Amplified Detection (APTIMA®) (0060243). Specimen must be collected and transported with test-specific kit.  Culture is recommended for Chlamydia trachomatis detection in suspected sexual abuse and for suspected failure of therapy. Refer to Chlamydia trachomatis Culture (Genital) (0060850).
CPT Code(s): 87270
Cross References: Chlamydia trachomatis, Fluorescence Staining of MicroTrak Slides (Chlamydia trachomatis DFA)