ARUP's Laboratory Test Directory

Amoeba Calcofluor Stain : 0060250

Mnemonic: ACANTSTAIN

Methodology: Qualitative Calcofluor Stain/Microscopy
Performed: Sun-Sat
Reported: Within 24 hours
Specimen Required: Collect: Corneal scrapings or vitreous fluid.

Specimen Preparation: Place corneal scrapings or vitreous fluid in 2 ml of Page's amoeba saline (ARUP supply #31917). Available online through eSupply using ARUP Connect™ or contact Client Services at (800) 522-2787.

Storage/Transport Temperature: Room temperature.

Unacceptable Conditions: Specimens in media or preservatives. Dry specimens.

Stability (collection to initiation of testing): Ambient: 72 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Reference Interval:
Negative
Interpretive Data: This stain will detect free-living amoeba such as Acanthamoeba species, Naegleria fowleri and Balamuthia mandrillaris.
Note: For CSF specimens refer to Acanthamoeba and Naegleria Culture and Stains, CSF (ARUP Test code 2002584). For Entamoeba histolytica Antigen, EIA (ARUP test code 0058001).
CPT Code(s): 87206
Cross References: Acanthamoeba Stain (Amoeba Calcofluor Stain) , Naegleria Stain (Amoeba Calcofluor Stain)