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0060153: Darkfield Exam

Time Sensitive

UUHSC Testing Only

Test Mnemonic: MS DRK
Methodology: Darkfield Microscopy Performed: Sun-Sat Reported: Within 24 hours

Specimen Required:

Collect: Fluid from abraded lesion or body fluid. Indicate source on test request form. Source of specimen is preferred. Testing performed for University of Utah Health Sciences Center only.

Transport: Fluid from abraded lesion or body fluid in a sterile container, sterile syringe, sealed microcapillary tube, or on a moist covered slide. Specimens must arrive at ARUP within 30 minutes from collection time at 20-25°C. Call ARUP's Bacteriology Laboratory at (801) 583-2787, ext. 2248 or 2169 to notify that specimen is being sent.

Submit specimen according to Biological Substance, Category B, shipping guidelines.

Pediatric Collection/Transport:

Submit specimen according to Biological Substance, Category B, shipping guidelines.

Remarks: Avoid drying or temperature extremes. Do not use soap or antibacterial agents for cleansing lesions. Spirochetes must be motile for presumptive identification to be made.

Unacceptable Conditions: Dry materials, delayed transport resulting in loss of motile organisms.

Stability: Ambient: 30 minutes; Refrigerated: Unacceptable; Frozen: Unacceptable

Reference Interval:

Negative for motile spirochetes.

CPT Code(s):
87166

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