ARUP's Laboratory Test Directory

Bordetella pertussis Culture : 0060117

Mnemonic: MC PERT

Methodology: Standard reference procedures for Bordetella pertussis culture and identification
Performed: Sun-Sat
Reported: Within 7 days
Preliminary: as soon as positive detected; Final: Negative at 7 days
Specimen Required: Collect: Nasopharyngeal swab, nasal aspirate or washing.
DFA is suggested in addition to culture. If both culture and DFA are ordered, two swabs or swab plus slides are required.


Specimen Preparation: Nasopharyngeal Swab: Place swab in Regan-Lowe transport media and incubate for 24-48 hours at 35°C (ARUP Supply# 24962). Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787 or send directly without incubation. Also acceptable: Place swab in Amies with charcoal media.
OR Aspirate or Washing:
Transfer to a sterile container.


Storage/Transport Temperature: Room temperature or refrigerated.

Submit specimen according to Biological Substance, Category B, shipping guidelines.


Remarks: Specimen source preferred.

Unacceptable Conditions: Sputum.

Stability (collection to initiation of testing): Incubated Regan-Lowe: Ambient: 4 days; Refrigerated: 4 days; Frozen: Unacceptable
Unincubated Regan-Lowe, Amies swab, Aspirate or Washing:
Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable

Reference Interval:
Culture negative for Bordetella pertussis.
Interpretive Data: A negative culture does not exclude the possibility of Bordetella pertussis infection.
Note: Bordetella pertussis and Bordetella parapertussis detection by PCR also available (0065080).
CPT Code(s): 87081 presumptive identification.  If definitive identification required, add 87077.
Cross References: Culture, Bordetella pertussis (Bordetella pertussis Culture) , Pertussis Culture (Bordetella pertussis Culture) , Whooping Cough Culture (Bordetella pertussis Culture)
 
 

 

 

 
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