ARUP's Laboratory Test Directory

0060117: Bordetella pertussis Culture

Test Mnemonic: MC PERT
Methodology: Standard reference procedures for Bordetella pertussis culture and identification

Performed: Sun-Sat

Reported: Within 7 days
Preliminary: as soon as positive detected; Final: Negative at 7 days

Specimen Required:  
Collect: Nasopharyngeal swab in Regan-Lowe transport media (preferred, ARUP Supply# 24962) or Nasopharyngeal swab in Amies with charcoal; nasal washing or aspirate in sterile container.  Source of specimen is preferred.

Transport: Leave swab in Regan-Lowe, incubate 24-48 hours at 35°C or send without incubation. Transport swab, media or washings ambient or refrigerated.

Submit specimen according to Biological Substance, Category B, shipping guidelines.

Pediatric Collection/Transport:

Submit specimen according to Biological Substance, Category B, shipping guidelines.

Remarks: DFA, in addition to culture, is suggested. If both culture and DFA are ordered, two swabs or swab plus slides are required. 

Unacceptable Conditions: Sputum.

Stability: Ambient: 2 days; Refrigerated: 2 days; Frozen: Unacceptable
Incubated Regan-Lowe: Ambient: 4 days; Refrigerated: 4 days; Frozen: Unacceptable

Reference Interval:
Culture negative for Bordetella pertussis.

Note:
Bordetella pertussis and Bordetella parapertussis detection by PCR also available (0065080).

CPT Code(s):
87081 presumptive identification.  If definitive identification required, add 87077.

 

 

 
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