ARUP's Laboratory Test Directory

Bordetella pertussis Culture : 0060117

Mnemonic: MC PERT

Methodology: Standard reference procedures for Bordetella pertussis culture and identification
Performed: Sun-Sat
Reported: Within 7 days
Preliminary: as soon as positive detected; Final: Negative at 7 days
Specimen Required: Collect:  Nasopharyngeal swab, in Amies with charcoal or Regan-Lowe transport media or nasal washing or aspirate in sterile container.  Source of specimen is preferred.

Transport:  Swab in Regan-Lowe (ARUP Supply# 24962), incubate 24-48 hours at 35°C or send directly without incubation. Transport swab in Aimies, media or washings ambient or refrigerated.

Submit specimen according to Biological Substance, Category B, shipping guidelines.


Pediatric Collection/Transport:  

Submit specimen according to Biological Substance, Category B, shipping guidelines.


Remarks:  DFA, in addition to culture, is suggested. If both culture and DFA are ordered, two swabs or swab plus slides are required. 

Unacceptable Conditions:  Sputum.

Stability:  Ambient: 2 days; Refrigerated: 2 days; Frozen: Unacceptable
Incubated Regan-Lowe: Ambient: 4 days; Refrigerated: 4 days; Frozen: Unacceptable

Reference Interval:
Culture negative for Bordetella pertussis.
Interpretive Data: A negative culture does not exclude the possibility of Bordetella pertussis infection.
Note: Bordetella pertussis and Bordetella parapertussis detection by PCR also available (0065080).
CPT Code(s): 87081 presumptive identification.  If definitive identification required, add 87077.
 
 

 

 

 
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