| Methodology: |
Direct Fluorescent Antibody Stain
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| Performed: |
Sun-Sat
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| Reported: |
Within 24 hours
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| Specimen Required: |
Collect: Nasopharyngeal swab in Regan-Lowe transport media (ARUP Supply# 24962) or Amies with charcoal.
Also acceptable: Nasal washing or aspirate in sterile container or two prepared slides from nasopharyngeal swab. Source of specimen is preferred.
Transport: Leave swab in Regan-Lowe, incubate 24-48 hours at 35°C or send without incubation. Transport prepared duplicate slides, swab, media or washings ambient or refrigerated.
Submit specimen according to Biological Substance, Category B, shipping guidelines.
Pediatric Collection/Transport:
Submit specimen according to Biological Substance, Category B, shipping guidelines.
Remarks: Culture, in addition to DFA, is suggested. If both culture and DFA are ordered, two swabs or swab plus slides are required. Preferred source is nasopharyngeal swab or washings/aspirates. Refer to test code 0060117 for culture instructions.
Unacceptable Conditions: Improper labeling of slide for DFA (slide, NOT slide container, must be labeled with patient name and identifying number), dry swabs. Sputum.
Stability: Specimen: Ambient: 2 days; Refrigerated: 2 days; Frozen: Unacceptable
Slides: Ambient: 1 week; Refrigerated: 1 week; Frozen: 1 month
Regan-Lowe: Ambient: 4 days; Refrigerated: 4 days; Frozen: Unacceptable
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