ARUP's Laboratory Test Directory

0060112: Legionella pneumophila DFA

Test Mnemonic: MS LEGION
Methodology: Direct Fluorescent Antibody Stain

Performed: Sun-Sat

Reported: Within 24 hours

Specimen Required:  
Collect: Respiratory tract secretions and aspirates, tissues, fluids, sputum, abscess material.  Source of specimen is required.

Transport: Two prepared slides at 20-25°C or fluid in a sterile container. If delay in transport (greater than 24 hours) freeze at -20°C or, preferably, -70°C and transport frozen.

Submit specimen according to Biological Substance, Category B, shipping guidelines.

Pediatric Collection/Transport:

Submit specimen according to Biological Substance, Category B, shipping guidelines.

Remarks: To prevent drying, submit specimen in sterile, nonbacteriostatic water. Do not use saline in specimen collection. Culture in addition to DFA is recommended (Legionella Species, Culture (0060113)). Client will be notified of positive DFA.

Unacceptable Conditions: Urine, stool, wounds, or other culture material from non-respiratory sites.  Dry specimens, nonsterile or leaking containers, or specimens submitted in saline, formalin, or viral transport medium.

Stability: Ambient: 2 hours; Refrigerated: 24 hours; Frozen: 1 week

Reference Interval:
Negative for Legionella pneumophila. 

Interpretive Data:
DFA is not recommended for diagnosing Legionella infections. If clinically indicated, please submit additional specimen for culture or nucleic acid amplification testing.



CPT Code(s):
87278

 

 

 
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