| Methodology: |
Direct Fluorescent Antibody Stain
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| Performed: |
Sun-Sat
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| Reported: |
Within 24 hours
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| Specimen Required: |
Collect: Respiratory tract secretions, aspirates, tissue, fluids, sputum, abscess material; pericardial fluid. Source of specimen is preferred.
Storage/Transport Temperature: Prepared duplicate slides or fluid in a sterile container at 20-25°C. If delay in transport (greater than 48 hours) transport frozen.
Submit specimen according to Biological Substance, Category B, shipping guidelines.
Remarks: To prevent drying, submit specimen in sterile, nonbacteriostatic water. Do not use saline in specimen collection. BAL fluids containing saline are acceptable. Culture in addition to DFA is recommended (Legionella Species, Culture (0060113)). Client will be notified of positive DFA.
Unacceptable Conditions: Urine, stool, wounds, or other culture material from non-respiratory sites. Dry specimens, nonsterile or leaking containers, or specimens submitted in saline, formalin, or viral transport medium.
Stability (collection to initiation of testing): Specimen: Ambient: 12 hours; Refrigerated: 48 hours; Frozen: 1 week
Slides: Ambient: 1 week; Refrigerated: 1 week; Frozen: 1 week
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