| Methodology: |
Transcription Mediated Amplification (Gen-Probe®)
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| Performed: |
Processed: Sun-Sat
Assayed: Mon, Wed, Fri
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| Reported: |
1-4 days
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| Specimen Required: |
Collect: Sputum, bronchoalveolar lavage (BAL), pleural fluid or other respiratory specimens in a sterile, leak-proof container. Respiratory specimen processed by NaOH-NALC digestion decontamination procedure. Unprocessed specimen is preferred. Source of specimen is required.
To perform this test it is essential to know whether or not the submitted specimen has been processed (digestion and decontamination procedure). If it has, smear results must be provided. Delayed turn around time will occur when the required information is not provided.
Storage/Transport Temperature: For unprocessed specimens: Submit 10-15 mL unprocessed respiratory specimen in a sterile leak-proof container at 2-8°C. Place each specimen in an individual sealed bag. (Min: 1 mL unprocessed specimen).
For processed specimens: Submit 5-10 mL processed respiratory specimen in a sterile leak-proof container at 2-8°C. Place each specimen in an individual sealed bag. (Min: 1 mL processed specimen) Identify processed specimens with method used for digestion. For processed specimen smear results must be provided.
Submit specimen according to Biological Substance, Category B, shipping guidelines.
Remarks: Collection of early-morning specimens of sputum on each of three consecutive days is optimum.
Unacceptable Conditions: Bloody specimens, leaking containers, and lung tissue. Alpha-Tec XPR-Plus is inhibitory (see Note below).
Stability (collection to initiation of testing): Ambient: Unacceptable; Refrigerated: Unprocessed specimen: 1 week; Processed specimen: 3 days; Frozen: 1 week
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