| Methodology: |
Transcription Mediated Amplification (Gen-Probe®)
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| Performed: |
Processed: Sun-Sat
Assayed: Mon, Wed, Fri
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| Reported: |
1-4 days
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| Specimen Required: |
Collect: Sputum, bronchoalveolar lavage (BAL), pleural fluid or other respiratory specimens in a sterile, leak-proof container. Respiratory specimen processed by NaOH-NALC digestion. Unprocessed specimen is preferred. Source of specimen is required.
Transport: For unprocessed specimens: Submit 10-15 mL unprocessed respiratory specimen in a sterile leak-proof container at 2-8°C. Place each specimen in an individual sealed bag. (Min: 1 mL unprocessed specimen).
For processed specimens: Submit 5-10 mL processed respiratory specimen in a sterile leak-proof container at 2-8°C. Place each specimen in an individual sealed bag. (Min: 1 mL processed specimen) Identify processed specimens with method used for digestion. For processed specimen smear results must be provided.
Submit specimen according to Biological Substance, Category B, shipping guidelines.
Pediatric Collection/Transport:
Submit specimen according to Biological Substance, Category B, shipping guidelines.
Remarks: Collection of early-morning specimens of sputum on each of three consecutive days is optimum.
Unacceptable Conditions: Bloody specimens, leaking containers, and lung tissue. Alpha-Tec XPR-Plus is inhibitory (see Note below).
Stability: Ambient: Unacceptable; Refrigerated: Unprocessed specimen: 1 week; Processed specimen: 3 days; Frozen: 1 week
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