ARUP's Laboratory Test Directory
| 0056025: Hepatitis B Virus DNA Ultrasensitive Quantitative Real-Time PCR |
| Test Mnemonic: HBVUPCR | |
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Methodology: Real-Time Polymerase Chain Reaction
Performed: Sun-Sat Reported: 2-4 days |
| Specimen Required: | |
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Collect: One 7 mL SST, PPT, lavender (EDTA), or pink (K2EDTA). (Min: 5 mL)
Transport: 2 mL serum or plasma, frozen. (Min: 0.5 mL) Submit specimen in an ARUP Standard Transport Tube. Specimens collected in a PPT should be submitted in original container. Submit specimen according to Biological Substance, Category B, shipping guidelines. Pediatric Collection/Transport: 0.25 mL serum or plasma, frozen. Submit specimen according to Biological Substance, Category B, shipping guidelines. Remarks: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered. Separate serum or plasma from cells within four hours. Unacceptable Conditions: Nonfrozen specimens or specimens exposed to repeated freeze/thaw cycles. Heparinized specimens. Polystyrene tubes. Stability: Ambient: 4 hours (on cells); Plasma or serum removed from cells within four hours can be refrigerated and frozen as follows: Refrigerated: 24 hours; Frozen: 4 months |
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| Reference Interval: |
| Not detected |
| Interpretive Data: | |
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The quantitative range of this assay is 1.6-8.3 log IU/mL (40-200,000,000 IU/mL). An interpretation of "Not Detected" does not rule out the presence of PCR inhibitors in the patient specimen or hepatitis B virus DNA concentrations below the level of detection of the assay. Care should be taken when interpreting any single viral load determination. In less than 1 percent of cases, low values (in the range of 1.6 to 2.8 log IU/mL) may be seen in specimens from patients who are uninfected. 1 IU/mL of HBV DNA is approximately 5 copies/mL. 1 IU/mL of HBV DNA is approximately 0.000018 pg/mL. This assay should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular Tissue-Based Products (HCT/P). Please refer to Statement A in the Compliance Statements section in the front of the Laboratory Test Directory. |
| Note: | |
| This test is intended for use in conjunction with clinical presentation and other laboratory markers as an indicator of disease prognosis. This test is also used as an aid in accessing viral response to treatment as measured by changes in HBV DNA levels. |
| CPT Code(s): | |
| 87517 |