| Methodology: |
Reverse Transcription Polymerase Chain Reaction
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| Performed: |
Varies
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| Reported: |
5-10 days
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| Specimen Required: |
Collect: Tissue.
Specimen Preparation: Freeze fresh tissue immediately. Transport 100 mg or 0.5 cm3 tissue.
OR Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Transport tissue block or 4 tissue scrolls (tissue shavings), 10 microns thick. Protect paraffin block from excessive heat. Tissue block will be returned after testing.
Transport tissue (frozen or paraffin block) in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Storage/Transport Temperature: Fresh tissue: Frozen at -70°C.
OR FFPE: Room temperature or refrigerated. Ship in cooled container during summer months.
Unacceptable Conditions: Specimens fixed/processed in alternative fixatives (alcohol, Prefer®) or heavy metal fixatives (B-4 or B-5). Slides. Decalcified tissue. Paraffin section slides.
Stability (collection to initiation of testing): Fresh tissue: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen at -70°C: 1 year
FFPE: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
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