ARUP's Laboratory Test Directory

BIRC2-MALT1 (API2-MALT1) Translocation, t(11;18) by RT-PCR : 0055691

Mnemonic: API2-MLT1

Methodology: Reverse Transcription Polymerase Chain Reaction
Performed: Sun-Sat
Reported: 5-10 days
Specimen Required: Collect: Tissue.

Specimen Preparation: Freeze fresh tissue immediately. Transport 100 mg or 0.5 cm3 tissue.
OR
Formalin fix (10% neutral buffered formalin) and paraffin embed tissue. Transport tissue block or 4 tissue scrolls (tissue shavings), 10 microns thick. Protect paraffin block from excessive heat.


Storage/Transport Temperature: Fresh tissue: Frozen at -70°C.
OR formalin-fixed, paraffin-embedded tissue:
Room temperature or refrigerated. Ship in cooled container during summer months.


Unacceptable Conditions: Specimens fixed/processed in alternative fixatives (alcohol, Prefer®) or heavy metal fixatives (B-4 or B-5). Slides. Decalcified tissue.

Stability (collection to initiation of testing): Fresh tissue: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen at -70°C: 48 hours
Formalin-fixed, paraffin-embedded tissue:
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable


Interpretive Data: Refer to report.

Refer to Statement B under Testing Information at http://www.aruplab.com.
Note: The chromosomal translocation t(11;18) or api2-mlt1 gene rearrangement is present in 40-50% of patients with MALT-type lymphomas of the stomach.  Since most api2-mlt1 breakpoints cluster at a common breakpoint, this RT-PCR assay is capable of detecting most of api2-mlt1 gene rearrangements.  However, some breakpoints occur at other loci and will not be identified by this particular test.
CPT Code(s): 83891 Isolation; 83902 Reverse transcription; 83898 x3 Amplification; 83894 Gel separation; 83912 Interpretation and report - Additional CPT code modifiers may be required for procedures performed to test for oncologic or inherited disorders.
Cross References: API2-MALT1 (BIRC2-MALT1 (API2-MALT1) Translocation, t(11;18) by RT-PCR)
 
 

 

 

 
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