Vitamin B[12] with Reflex to Methylmalonic Acid, Serum (Vitamin B[12] Deficiency)

ARUP's Laboratory Test Directory

Vitamin B₁₂ with Reflex to Methylmalonic Acid, Serum (Vitamin B₁₂ Deficiency) : 0055662

Mnemonic: B12 MMA

Ordering Recommendation:	Preferred reflex test for detection of vitamin B₁₂ deficiency in individuals with macrocytic or unexplained anemia, or unexplained neurologic disease.
Methodology:	Quantitative Chemiluminescent Immunoassay/Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry
Performed:	Sun-Sat
Reported:	1-3 days
Specimen Required:	Collect: Plain red or serum separator tube. Also acceptable: Green (sodium heparin), lavender (EDTA), or pink (K₂EDTA). Specimen Preparation: Protect from light during collection, storage and shipment. Centrifuge and remove serum from cells within 2 hours of collection. Transfer 2 mL serum to an ARUP Standard Transport Tube and refrigerate or freeze immediately. (Min: 1.5 mL) Storage/Transport Temperature: Frozen. Unacceptable Conditions: Room temperature specimens. Grossly hemolyzed or lipemic specimens. Stability (collection to initiation of testing): After separation from cells: Ambient: Unacceptable; Refrigerated: 48 hours; Frozen: 1 month

Reference Interval:

Test Number	Components	Reference Interval
0070150	Vitamin B₁₂	210-911 pg/mL
	Methylmalonic Acid, Serum (Vitamin B₁₂ Deficiency)	0.00-0.40 µmol/L

Note:

If Vitamin B₁₂ is less than 300 pg/mL, then Methylmalonic Acid, Serum (Vitamin B₁₂Deficiency) will be added. Additional charges apply.

CPT Code(s):

82607; if reflexed, add 83921

Cross References:

B₁₂ (Cobalamin) (Vitamin B₁₂ with Reflex to Methylmalonic Acid, Serum (Vitamin B₁₂ Deficiency))

Testing Information