ARUP's Laboratory Test Directory

0055619: bcl-2/JH, t(14;18) Translocation by PCR, Tissue

Test Mnemonic: BCL2C-T
Methodology: Polymerase Chain Reaction

Performed: DNA isolation: Sun-Sat; Assay: Varies

Reported: 2-7 days

Specimen Required:  
Collect: Fresh tissue. Freeze immediately.

Transport: 100 mg or 0.5-2.0 cm3 tissue, frozen on dry ice.

Remarks: Formalin-fixed, paraffin-embedded tissue can be submitted, but it is not an optimal specimen source.

Stability: Ambient: Unacceptable; Refrigerated: 2 hours; Frozen: 1 year

Reference Interval:
 
Test Number Components Reference Interval
 bcl-2/JH, t(14;18) Translocation Major Breakpoint Region (MBR), Tissue Negative: bcl-2/JH major breakpoint region gene rearrangement is not detected.
Positive: bcl-2/JH major breakpoint region gene rearrangement is detected.
 bcl-2/JH, t(14;18) Translocation Minor Cluster Region (MCR), Tissue Negative: bcl-2/JH minor cluster region gene rearrangement is not detected.
Positive: bcl-2/JH minor cluster region gene rearrangement is detected.

Interpretive Data:
By report

Please refer to Statement B in the Compliance Statements section in the front of the Laboratory Test Directory.



Note:
A positive result indicates the presence of a bcl-2/JH t(14;18) chromosomal translocation.  A negative result does not entirely exclude the presence of a bcl-2/JH chromosomal t(14;18) translocation.

Comment: The chromosomal translocation t(14;18) or bcl-2 gene rearrangement is present in approximately 90% of follicular lymphomas and in a significant minority (10-30%) of diffuse large-cell lymphomas of B-cell lineage. Since most bcl-2 breakpoints cluster at the major breakpoint region (~60%) or minor cluster region (~10%), this PCR assay is capable of detecting a majority of bcl-2/JH gene rearrangements. However, some breakpoints occur at distant loci and will not be identified by this particular test.

CPT Code(s):
83891 Isolation; 83898 x6 Amplification; 83894 x3 Gel separation; 83912 Interpretation and report - Additional CPT code modifiers may be required for procedures performed to test for oncologic or inherited disorders.