ARUP's Laboratory Test Directory
| 0055616: bcl-2/JH, t(14;18) Translocation by PCR, Fluid |
| Test Mnemonic: BCL2C-F | |
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Methodology: Polymerase Chain Reaction
Performed: DNA isolation: Sun-Sat; Assay: Sun-Sat Reported: 2-7 days |
| Specimen Required: | |
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Collect: One 5 mL lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A), or 3 mL bone marrow (lavender, EDTA or pink K2EDTA).
Transport: 5 mL whole blood or 3 mL bone marrow at 2-8°C. (Min: 3 mL) Pediatric Collection/Transport: 1 mL whole blood or bone marrow at 2-8°C. Remarks: CSF and pleural fluid are also acceptable. Unacceptable Conditions: Serum, frozen whole blood, or clotted blood. Severely hemolyzed specimens. Stability: Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable |
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| Interpretive Data: | |
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By report
Please refer to Statement B in the Compliance Statements section in the front of the Laboratory Test Directory. |
| Note: | |
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A positive result indicates the presence of a bcl-2/JH t(14;18) chromosomal translocation. A negative result does not entirely exclude the presence of a bcl-2/JH chromosomal t(14;18) translocation. Comment: The chromosomal translocation t(14;18) or bcl-2 gene rearrangement is present in approximately 90% of follicular lymphomas and in a significant minority (10-30%) of diffuse large-cell lymphomas of B-cell lineage. Since most bcl-2 breakpoints cluster at the major breakpoint region (~60%) or minor cluster region (~10%), this PCR assay is capable of detecting a majority of bcl-2/JH gene rearrangements. However, some breakpoints occur at distant loci and will not be identified by this particular test. |
| CPT Code(s): | |
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83891 Isolation; 83898 x6 Amplification; 83894 x3 Gel separation; 83912 Interpretation and report - Additional CPT code modifiers may be required for procedures performed to test for oncologic or inherited disorders. |