ARUP's Laboratory Test Directory
| 0055596: T-Cell Clonality Assessment by Restriction Fragment-Southern Blot Hybridization, Fluid |
| Test Mnemonic: TCRFSBHF | |
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Methodology: Restriction Fragment Southern Blot Hybridization
Performed: DNA isolation: Sun-Sat; Assay: Varies Reported: 7-14 days |
| Specimen Required: | |
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Collect: Two 5 mL lavender (EDTA) or pink (K2 EDTA), one 10 mL yellow (ACD), or 3 mL bone marrow.
Transport: 10 mL whole blood or 3 mL bone marrow (EDTA) at 2-8°C. (Min: 3 mL) Pediatric Collection/Transport: 3 mL whole blood or 1 mL bone marrow (EDTA) at 2-8°C. Unacceptable Conditions: Serum, frozen whole blood, clotted blood, and severely hemolyzed samples. Stability: Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable |
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| Reference Interval: |
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Negative: A monoclonal T-cell population is not detected. Positive: A monoclonal T-cell population is detected. (Monoclonal T-cell receptor beta rearrangement identified by Southern blot analysis.) |
| Interpretive Data: | |
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By report
Please refer to Statement B in the Compliance Statements section in the front of the Laboratory Test Directory. |
| Note: | |
| A positive result indicates the presence of monoclonal T-cell population within the sample. A negative result does not entirely exclude the presence of a T-cell receptor gene rearrangement (or monoclonal T-cell population) in the sample. |
| CPT Code(s): | |
| 83891 Isolation; 83892 x3 Digestion; 83894 Gel Electrophoresis; 83897 Southern transfer; 83896 Nucleic acid probe; 83912 Interpretation and report - Additional CPT code modifiers may be required for procedures performed to test for oncologic or inherited disorders. |