ARUP's Laboratory Test Directory

0055594: T-Cell Clonality Assessment by Restriction Fragment-Southern Blot Hybridization, Tissue

Test Mnemonic: TCRFSBHT
Methodology: Restriction Fragment Southern Blot Hybridization

Performed: DNA isolation: Sun-Sat; Assay: Varies

Reported: 7-14 days

Specimen Required:  
Collect: Fresh tissue.  Freeze immediately.

Transport: 100 mg or 0.5-2.0 cm3 tissue, frozen on dry ice.

Unacceptable Conditions: Paraffin samples.

Stability: Ambient: Unacceptable; Refrigerated: 2 hours; Frozen: 1 year

Reference Interval:
Negative: A monoclonal T-cell population is not detected.
Positive: A monoclonal T-cell population is detected.  (Monoclonal T-cell receptor beta gene rearrangement identified by Southern blot analysis.)

Interpretive Data:
By report

Please refer to Statement B in the Compliance Statements section in the front of the Laboratory Test Directory.



Note:
A positive result indicates the presence of monoclonal T-cell population within the sample.  A negative result does not entirely exclude the presence of a T-cell receptor gene rearrangement (or monoclonal T-cell population) in the sample.

CPT Code(s):
83907 Lysis; 83891 Isolation; 83892 x3 Digestion; 83894 Gel electrophoresis; 83897 Southern transfer; 83896 Nucleic acid probe; 83912 Interpretation and report - Additional CPT code modifiers may be required for procedures performed to test for oncologic or inherited disorders.