| Ordering Recommendation: |
Not a first-line test for Lyme disease. May be useful if strong suspicion of Lyme disease persists in spite of persistent negative serologic testing.
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| Methodology: |
Qualitative Polymerase Chain Reaction
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| Performed: |
Mon, Wed, Fri
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| Reported: |
1-4 days
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| Specimen Required: |
Collect: Lavender (EDTA), pink (K2EDTA) or serum separator tube. OR CSF, synovial fluid or tissue.
Specimen Preparation: Separate serum or plasma from cells. Transfer 1 mL serum, plasma, CSF or synovial fluid to a sterile container. (Min: 0.5 mL). Tissue: Transfer to a sterile container and freeze immediately.
Storage/Transport Temperature: Frozen.
Remarks: Specimen source required.
Unacceptable Conditions: Heparinized specimens.
Stability (collection to initiation of testing): Tissue: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 1 year
All Others: Ambient: 8 hours; Refrigerated: 72 hours; Frozen: 1 year
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