ARUP's Laboratory Test Directory

T-Cell Clonality Screening Assay by PCR, Fluid : 0055567

Mnemonic: T CELL-F

Methodology: Polymerase Chain Reaction/Fragment Analysis
Performed: DNA isolation: Sun-Sat; Assay: Varies
Reported: 7-10 days
Specimen Required: Collect:  Two 5 mL lavender (EDTA) or pink (K2 EDTA), one 10 mL yellow (ACD), or 3 mL bone marrow. (Min: 3 mL EDTA or yellow)

Transport:  10 mL whole blood or 3 mL bone marrow (EDTA) at 2-8°C. (Min: 3 mL)

Pediatric Collection/Transport:  1 mL whole blood or 1 mL bone marrow (EDTA) at 2-8°C.

Remarks:  Also acceptable: CSF and pleural fluid.

Unacceptable Conditions:  Serum, frozen whole blood, clotted blood, and severely hemolyzed samples.

Stability:  Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable
Reference Interval:
Negative: A monoclonal T-cell population is not detected.
Positive: A monoclonal T-cell population is detected.
Interpretive Data: By report

Please refer to Statement B in the Compliance Statements section in the front of the Laboratory Test Directory.
Note: A positive result indicates the presence of a monoclonal T-cell population within the sample.  A negative result does not entirely exclude the presence of a T-cell receptor gamma rearrangement (or monoclonal T-cell population) in the sample. Negative PCR screening studies should be terminally analyzed by restriction fragment Southern blot hybridization (RF-SBH) to definitively exclude T-cell monoclonality.  This is not automatically performed unless otherwise stipulated.

For confirmation order T-Cell Clonality Assessment by Restriction Fragment Southern Blot Hybridization, Fluid (0055596).
CPT Code(s): 83891 Isolation; 83898 x6 Amplification; 83896 x3 Nucleic acid probes; 83909 Separation and identification; 83912 Interpretation and report  - Additional CPT code modifiers may be required for procedures performed to test for oncologic or inherited disorders.
 
 

 

 

 
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