ARUP's Laboratory Test Directory

T-Cell Clonality Screening by PCR : 0055567

Mnemonic: T CELL-F

Methodology: Polymerase Chain Reaction/Capillary Electrophoresis
Performed: DNA isolation: Sun-Sat; Assay: Varies
Reported: 5-7 days
Specimen Required: Collect: Lavender (EDTA) or bone marrow (EDTA) OR tissue.

Specimen Preparation: Whole blood: Transport 5 mL. (Min: 1 mL)
OR Bone marrow:
Transport 3 mL. (Min: 1 mL)
OR Fresh tissue:
Freeze immediately. Transport 100 mg or 0.5-2.0 cm3 tissue
OR FFPE tumor tissue:
Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Transport tissue block or four 10-micron shavings. Tissue block will be returned after testing. Transport tissue (frozen or paraffin) in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.


Storage/Transport Temperature: Whole blood OR Bone marrow: Refrigerated.
Fresh tissue:
Frozen on dry ice.
FFPE tumor tissue:
Room temperature or refrigerated. Ship in cooled container during summer months.


Unacceptable Conditions: Serum. Frozen whole blood or bone marrow. Clotted or grossly hemolyzed specimens. Specimens fixed/processed in alternative fixatives or heavy metal fixatives (B-4 or B-5) or tissue sections on slides. Decalcified specimens.

Stability (collection to initiation of testing): Whole blood OR Bone marrow: Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable
Fresh Tissue:
Ambient: Unacceptable; Refrigerated: 2 hours; Frozen: 1 year
FFPE tumor tissue:
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable


Interpretive Data: Refer to report.

See Compliance Statement B: www.aruplab.com/CS
CPT Code(s): 81342, G0452
Cross References: T Cell Gamma (T-Cell Clonality Screening Assay by PCR, Fluid), T Cell Gene Rearrangement (T-Cell Clonality Screening Assay by PCR, Fluid), TCRG (T-Cell Clonality Screening Assay by PCR, Fluid)